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Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor

文献类型：期刊论文

作者Hu, Liang1,2,3; Ge, Anle1,2,4,5; Wang, Xixian1,2,4,5; Wang, Shanshan1,2; Yue, Xinpei6; Wang, Jie1,2; Feng, Xiaojun1,2; Du, Wei1,2; Liu, Bi-Feng1,2
刊名BIOSENSORS & BIOELECTRONICS
出版日期2018-07-01
卷号110页码:233-238
关键词Microfluidic Chip Worm-based Biosensor Immune Responses In Vivo Analysis Drug Evaluation
ISSN号0956-5663
DOI10.1016/j.bios.2018.03.058
文献子类Article
英文摘要Immune response to environmental pathogen invasion is a complex process to prevent host from further damage. For quantitatively understanding immune responses and revealing the pathogenic environmental information, real-time monitoring of such a whole dynamic process with single-animal resolution in well-defined environments is highly desired. In this work, an integrated microfluidic device coupled with worm-based biosensor was proposed for in vivo studies of dynamic immune responses and antibiotics interference in infected C. elegans. Individual worms housed in chambers were exposed to the various pathogens and discontinuously manipulated for imaging with limited influence on physiological activities. The expression of immune responses gene (irg-1) was time-lapse measured in intact worms during pathogen infection. Results demonstrated that irg-1 gene could be induced in the presence of P. aeruginosa strain PA14 in a dose-dependent manner, and the survival of infected worm could be rescued under gentamicin or erythromycin treatments. We expect it to be a versatile platform to facilitate future studies on pathogenesis researches and rapid drug screen using C. elegans disease model.
WOS关键词ANIMAL INFECTION MODEL ; C.-ELEGANS MODEL ; CAENORHABDITIS-ELEGANS ; IN-VIVO ; PSEUDOMONAS-AERUGINOSA ; INNATE IMMUNITY ; NEURONAL-ACTIVITY ; CHIP ; ERYTHROMYCIN ; DISCOVERY
WOS研究方向Biophysics ; Biotechnology & Applied Microbiology ; Chemistry ; Electrochemistry ; Science & Technology - Other Topics
语种英语
WOS记录号WOS:000432613300031
出版者ELSEVIER ADVANCED TECHNOLOGY
资助机构National Key R&D Program of China(2016YFF0100801) ; National Natural Science Foundation of China(21475049 ; Fundamental Research Funds for the Central Universities(2016YXZD061) ; 31471257 ; 21275060)
源URL[http://ir.qibebt.ac.cn/handle/337004/11429]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Du, Wei; Liu, Bi-Feng
作者单位1.Huazhong Univ Sci & Technol, Key Lab Biomed Photon, MOE, Wuhan Natl Lab Optoelect Hubei Bioinformat, Wuhan 430074, Hubei, Peoples R China
2.Huazhong Univ Sci & Technol, Mol Imaging Key Lab, Syst Biol Theme, Dept Biomed Engn,Coll Life Sci & Technol, Wuhan 430074, Hubei, Peoples R China
3.Chinese Acad Sci, Wuhan Inst Phys & Math, Brain Res Ctr, Wuhan 430071, Hubei, Peoples R China
4.Chinese Acad Sci, CAS Key Lab Biofuels, Single Cell Ctr, Qingdao 266101, Shandong, Peoples R China
5.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, Shandong Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
6.Wuhan Univ Technol, Sch Chem Chem Engn & Life Sci, Wuhan 430070, Hubei, Peoples R China
推荐引用方式
GB/T 7714		 Hu, Liang,Ge, Anle,Wang, Xixian,et al. Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor[J]. BIOSENSORS & BIOELECTRONICS,2018,110:233-238.
APA Hu, Liang.,Ge, Anle.,Wang, Xixian.,Wang, Shanshan.,Yue, Xinpei.,...&Liu, Bi-Feng.(2018).Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor.BIOSENSORS & BIOELECTRONICS,110,233-238.
MLA Hu, Liang,et al."Real-time monitoring of immune responses under pathogen invasion and drug interference by integrated microfluidic device coupled with worm-based biosensor".BIOSENSORS & BIOELECTRONICS 110(2018):233-238.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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