Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis
文献类型：期刊论文
| 作者 | Chen, Hailin1,2; Li, Meijie1; Liu, Changqing1; Zhang, Haibo1 ; Xian, Mo1; Liu, Huizhou1
|
| 刊名 | MICROBIAL CELL FACTORIES
 |
| 出版日期 | 2018-04-30 |
| 卷号 | 17 |
| 关键词 | Isopentenyl Diphosphate Isomerase Random Mutagenesis Enzyme Activity Lycopene Production Mevalonate Pathway |
| ISSN号 | 1475-2859 |
| DOI | 10.1186/s12934-018-0913-z |
| 文献子类 | Article |
| 英文摘要 | Background: Lycopene is a terpenoid pigment that has diverse applications in the food and medicine industries. A prospective approach for lycopene production is by metabolic engineering in microbial hosts, such as Escherichia coli. Isopentenyl diphosphate isomerase (IDI, E.C. 5.3.3.2) is one of the rate-limiting enzymes in the lycopene biosynthetic pathway and one major target during metabolic engineering. The properties of IDIs differ depending on the sources, but under physiological conditions, IDIs are limited by low enzyme activity, short half-life and weak substrate affinity. Therefore, it is important to prepare an excellent IDI by protein engineering. |
| WOS关键词 | MEVALONATE-INDEPENDENT PATHWAY ; DIMETHYLALLYL DIPHOSPHATE ; ESCHERICHIA-COLI ; STREPTOCOCCUS-PNEUMONIAE ; ISOPRENOID BIOSYNTHESIS ; CRYSTAL-STRUCTURE ; LYCOPENE ; EVOLUTION ; CHLOROPLASTS ; INHIBITION |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000431339000002 |
| 出版者 | BIOMED CENTRAL LTD |
| 资助机构 | National Natural Science Foundation of China(21572242) ; Taishan Scholars Climbing Program of Shandong(tspd20150210) ; National Natural Science Foundation of China (NSF)(31400084) ; Youth Innovation Promotion Association CAS(2017252) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11483]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Zhang, Haibo; Xian, Mo |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Univ Chinese Acad Sci, Sino Danish Coll, 19 A Yuquan Rd, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Hailin,Li, Meijie,Liu, Changqing,et al. Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis[J]. MICROBIAL CELL FACTORIES,2018,17. |
| APA | Chen, Hailin,Li, Meijie,Liu, Changqing,Zhang, Haibo,Xian, Mo,&Liu, Huizhou.(2018).Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis.MICROBIAL CELL FACTORIES,17. |
| MLA | Chen, Hailin,et al."Enhancement of the catalytic activity of Isopentenyl diphosphate isomerase (IDI) from Saccharomyces cerevisiae through random and site-directed mutagenesis".MICROBIAL CELL FACTORIES 17(2018). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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