中国科学院机构知识库网格
Chinese Academy of Sciences Institutional Repositories Grid
首页 机构 成果 学者
  
  1. CAS IR Grid
  2. 青岛生物能源与过程研究所
  3. 中国科学院青岛生物能源与过程研究所
First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF

文献类型：期刊论文

作者Gu, Han-jie1,2,3,4; Sun, Qing-lei1,2,3; Jiang, Shuai1,2,3; Zhang, Jian1,2,3,5; Sun, Li1,2,3
刊名DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY
出版日期2018-07-01
卷号84页码:382-395
关键词Anti-lipopolysaccharide Factor Antimicrobial Peptides Rimicaris Sp. Deep-sea Hydrothermal Vent Antibacterial Antiviral
ISSN号0145-305X
DOI10.1016/j.dci.2018.03.016
文献子类Article
英文摘要Anti-lipopolysaccharide factor (ALF) is a type of antimicrobial peptides (AMPs) with a vital role in antimicrobial defense. Although a large amount of ALFs have been identified from neritic and fresh water crustacean species, no functional investigation of ALFs from deep-sea animals have been documented. In the present study, we characterized the immune function of an ALF molecule (named RspALF1) from the shrimp Rimicaris sp. residing in the deep-sea hydrothermal vent in Desmos, Manus Basin. RspALF1 shares 51.5%-62.4% overall sequence identities with known shrimp ALFs and contains the conserved LPS binding domain (LBD). Both recombinant RspALF1 (rRspALF1) and the LBD-derived peptide (ALF1P1) bound to the cell wall components of Gram-negative and Gram-positive bacteria and killed a wide range of bacteria, especially those from deep-sea hydrothermal field, by damaging bacterial cellular structures. The bactericidal activities of rRspALF1 and ALF1P1 were optimal and stably maintained from 4 C to 37 C, which is comparable to the ambient temperature range of the habitat of Rimicaris sp. In addition to bacteria, rRspALF1 and ALF1P1 also exhibited anti-fungal activity. rRspALF1 and ALF1P1 exhibited high killing efficiencies, which, in terms of MIC values, were ranged between 0.25 M and 4 M for bacteria and 4 mu M-81.1 mu M for fungi. When introduced in vivo, both rRspALF1 and ALF1P1 effectively inhibited bacterial infection in shrimp and reduced the dissemination of bacterial and viral pathogens in fish. Together, these results provide the first insight into the biological property of deep-sea ALF and indicate that RspALF1 very likely plays a significant role in immune defense by functioning as a highly effective antimicrobial with a broad target range. (C) 2018 Elsevier Ltd. All rights reserved.
WOS关键词ANTI-LPS FACTOR ; BLACK TIGER SHRIMP ; CRAB SCYLLA-PARAMAMOSAIN ; ANTIMICROBIAL PEPTIDES ; PENAEUS-MONODON ; MUD CRAB ; MOLECULAR-CLONING ; BINDING DOMAIN ; MARSUPENAEUS-JAPONICUS ; GENOMIC ORGANIZATION
WOS研究方向Fisheries ; Immunology ; Zoology
语种英语
WOS记录号WOS:000431160800037
出版者ELSEVIER SCI LTD
资助机构Strategic Priority Research Program of Chinese Academy of Sciences(XDA11030401) ; Scientific and Technological Innovation Project - Qingdao National Laboratory for Marine Science and Technology(2016ASKJ14) ; AoShan Talents Program by Qingdao National Laboratory for Marine Science and Technology(2015ASTP) ; Taishan Scholar Program of Shandong Province
源URL[http://ir.qibebt.ac.cn/handle/337004/11497]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Zhang, Jian; Sun, Li
作者单位1.Chinese Acad Sci, CAS Key Lab Expt Marine Biol, Inst Oceanol, Qingdao, Peoples R China
2.Chinese Acad Sci, Ctr Ocean Mega Sci, Qingdao, Peoples R China
3.Qingdao Natl Lab Marine Sci & Technol, Lab Marine Biol & Biotechnol, Qingdao, Peoples R China
4.Univ Chinese Acad Sci, Beijing, Peoples R China
5.Chinese Acad Sci, Ctr Deep Sea Res, Inst Oceanol, Qingdao, Peoples R China
推荐引用方式
GB/T 7714		 Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,et al. First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF[J]. DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,2018,84:382-395.
APA Gu, Han-jie,Sun, Qing-lei,Jiang, Shuai,Zhang, Jian,&Sun, Li.(2018).First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF.DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY,84,382-395.
MLA Gu, Han-jie,et al."First characterization of an anti-lipopolysaccharide factor (ALF) from hydrothermal vent shrimp: Insights into the immune function of deep-sea crustacean ALF".DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY 84(2018):382-395.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

浏览0
下载0
收藏0
其他版本

除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。