Recent advances in the selection and identification of antigen-specific nanobodies
文献类型：期刊论文
| 作者 | Liu, Wenshuai1; Song, Haipeng2; Chen, Quan1; Yu, Jianli2; Xian, Mo1 ; Nian, Rui1; Feng, Dongxiao3
|
| 刊名 | MOLECULAR IMMUNOLOGY
 |
| 出版日期 | 2018-04-01 |
| 卷号 | 96页码:37-47 |
| 关键词 | Nanobody Vhh Antigen-specific Display Technology Library |
| ISSN号 | 0161-5890 |
| DOI | 10.1016/j.molimm.2018.02.012 |
| 文献子类 | Review |
| 英文摘要 | Nanobodies represent the next-generation antibody-derived biologics with significant advances over conventional antibodies. Several rapid and robust techniques for isolating highly specific nanobodies have been developed. Antigen specific nanobodies are selected from constructed nanobody libraries, which can be classified into 3 main types: immune library, naive library, and semisynthetic/synthetic library. The immune library is the most widely used strategy for nanobody screening. Target specific nanobodies are highly enriched in immune libraries than in non-immune libraries; however, it is largely limited by the natural antigenicity of antigens. The naive library is thus developed. Despite the lack of somatic maturation, protein engineering can be employed to significantly increase the affinities of selected binders. However, a substantial amount of blood samples collected from a large number of individual animals is a prerequisite to ensure the diversity of the naive library. With this issue considered, the semisynthetic/synthetic library may be a promising path toward obtaining a limitless source of nanobodies against a variety of antigens without the need of animals. In this review, we summarize the state-of-the-art screening technologies with different libraries. The approaches presented here can further boost the diverse applications of nanobodies in biomedicine and biotechnology. |
| WOS关键词 | SINGLE-DOMAIN ANTIBODY ; HEAVY-CHAIN ANTIBODIES ; IN-VITRO ; RIBOSOME-DISPLAY ; AFFINITY MATURATION ; PHAGE DISPLAY ; FUNCTIONAL PROTEINS ; SURFACE DISPLAY ; RAPID ISOLATION ; MICROCYSTIN-LR |
| WOS研究方向 | Biochemistry & Molecular Biology ; Immunology |
| 语种 | 英语 |
| WOS记录号 | WOS:000430647700005 |
| 出版者 | PERGAMON-ELSEVIER SCIENCE LTD |
| 资助机构 | Nanobodi-QIBEBT Collaboration Project(20163702021237) ; National Natural Science Foundation of China(21676286 ; Primary Research Development Plan of Shandong Province(2016GSF121006) ; 81702085) |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/11509]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Nian, Rui; Feng, Dongxiao |
| 作者单位 | 1.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biobased Mat, 189 Songling Rd, Qingdao 266101, Peoples R China 2.Shenzhen Innova Nanobodi Co Ltd, 7018 Caitian Rd, Shenzhen 518000, Peoples R China 3.Binzhou Med Univ, Sch Pharmaceut Sci, 346 Guanhai Rd, Yantai 264003, Peoples R China |
| 推荐引用方式 GB/T 7714 | Liu, Wenshuai,Song, Haipeng,Chen, Quan,et al. Recent advances in the selection and identification of antigen-specific nanobodies[J]. MOLECULAR IMMUNOLOGY,2018,96:37-47. |
| APA | Liu, Wenshuai.,Song, Haipeng.,Chen, Quan.,Yu, Jianli.,Xian, Mo.,...&Feng, Dongxiao.(2018).Recent advances in the selection and identification of antigen-specific nanobodies.MOLECULAR IMMUNOLOGY,96,37-47. |
| MLA | Liu, Wenshuai,et al."Recent advances in the selection and identification of antigen-specific nanobodies".MOLECULAR IMMUNOLOGY 96(2018):37-47. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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