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Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize

文献类型：期刊论文

作者Wu, Zhenying1,2; Ren, Hao3; Xiong, Wangdan1,2; Roje, Sanja4; Liu, Yuchen1,2; Su, Kunlong1,2; Fu, Chunxiang1,2
刊名JOURNAL OF EXPERIMENTAL BOTANY
出版日期2018-07-20
卷号69期号:16页码:3963-3973
关键词Lignin maize methylenetetrahydrofolate reductase methyl metabolism S-adenosyl homocysteine S-adenosyl methionine
ISSN号0022-0957
DOI10.1093/jxb/ery208
英文摘要The brown midrib2 (bm2) mutant of maize, which has a modified lignin composition, contains a mutation in the methylenetetrahydrofolate reductase (MTHFR) gene. Here, we show that a MITE transposon insertion caused downregulation of MTHFR, with an accompanying decrease in 5-methyl-tetrahydrofolate and an increase in 5, 10-methylene-tetrahydrofolate and tetrahydrofolate in the bm2 mutant. Furthermore, MTHFR mutation did not change the content of S-adenosyl methionine (SAM), the methyl group donor involved in the biosynthesis of guaiacyl and syringyl lignins, but increased the level of S-adenosyl homocysteine (SAH), the demethylation product of SAM. Moreover, competitive inhibition of the maize caffeoyl CoA O-methyltransferase (CCoAOMT) and caffeic acid O-methyltransferase (COMT) enzyme activities by SAH was found, suggesting that the SAH/SAM ratio, rather than the concentration of SAM, regulates the transmethylation reactions of lignin intermediates. Phenolic profiling revealed that caffeoyl alcohol glucose derivatives accumulated in the bm2 mutant, indicating impaired 3-O-methylation of monolignols. A remarkable increase in the unusual catechyl lignin in the mutant demonstrates that MTHFR down-regulation mainly affects guaiacyl lignin biosynthesis, consistent with the observation that CCoAOMT is more sensitive to SAH inhibition than COMT. This study uncovered a novel regulatory mechanism in lignin biosynthesis, which may offer an effective approach to utilizing lignocellulosic feedstocks in the future.
WOS关键词ACID O-METHYLTRANSFERASE ; ONE-CARBON METABOLISM ; BROWN-MIDRIB MUTANTS ; S-ADENOSYLMETHIONINE ; HIGHER-PLANTS ; GENE ENCODES ; BIOSYNTHESIS ; MUTATION ; ARABIDOPSIS ; LIGNIFICATION
资助项目National Key Technologies Research & Development Program-Seven Major Crops Breeding Project[2016YFD0101803] ; 100-Talent Program of the Chinese Academy of Sciences Foundation ; Natural Science Foundation of China[31470390]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000439796700014
出版者OXFORD UNIV PRESS
资助机构National Key Technologies Research & Development Program-Seven Major Crops Breeding Project ; 100-Talent Program of the Chinese Academy of Sciences Foundation ; Natural Science Foundation of China
源URL[http://ir.qibebt.ac.cn/handle/337004/11896]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Fu, Chunxiang
作者单位1.Chinese Acad Sci, Qingdao Engn Res Ctr Biomass Resources & Environm, Shandong Prov Key Lab Energy Genet, Qingdao 266101, Shandong, Peoples R China
2.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Shandong, Peoples R China
3.Nanjing Forestry Univ, Coll Light Ind & Food Engn, Nanjing 210037, Jiangsu, Peoples R China
4.Washington State Univ, Inst Biol Chem, Pullman, WA 99164 USA
推荐引用方式
GB/T 7714		 Wu, Zhenying,Ren, Hao,Xiong, Wangdan,et al. Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize[J]. JOURNAL OF EXPERIMENTAL BOTANY,2018,69(16):3963-3973.
APA Wu, Zhenying.,Ren, Hao.,Xiong, Wangdan.,Roje, Sanja.,Liu, Yuchen.,...&Fu, Chunxiang.(2018).Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize.JOURNAL OF EXPERIMENTAL BOTANY,69(16),3963-3973.
MLA Wu, Zhenying,et al."Methylenetetrahydrofolate reductase modulates methyl metabolism and lignin monomer methylation in maize".JOURNAL OF EXPERIMENTAL BOTANY 69.16(2018):3963-3973.

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来源：青岛生物能源与过程研究所

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