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Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum

文献类型：期刊论文

作者Li, Renmin1,2; Feng, Yingang1; Liu, Shiyue1,2; Qi, Kuan1,2; Cui, Qiu1; Liu, Ya-Jun1
刊名MICROBIAL BIOTECHNOLOGY
出版日期2018-09-01
卷号11期号:5页码:905-916
ISSN号1751-7915
DOI10.1111/1751-7915.13293
英文摘要Cellulosome is a highly efficient supramolecular machine for lignocellulose degradation, and its substrate-coupled regulation requires soluble transmembrane signals. However, the inducers for cellulosome synthesis and the inducing effect have not been clarified quantitatively. Values of cellulosome production capacity (CPC) and estimated specific activity (eSA) were calculated based on the primary scaffoldin ScaA to define the stimulating effects on the cellulosome synthesis in terms of quantity and quality respectively. The estimated cellulosome production of Clostridium thermocellum on glucose was at a low housekeeping level. Both Avicel and cellobiose increased CPCs of the cells instead of the eSAs of the cellulosome. The CPC of Avicel-grown cells was over 20-fold of that of glucose-grown cells, while both Avicel- and glucose-derived cellulosomes showed similar eSA. The CPC of cellobiose-grown cells was also over three times higher than glucose-grown cells, but the eSA of cellobiose-derived cellulosome was 16% lower than that of the glucose-derived cellulosome. Our results indicated that cello-oligosaccharides played the key roles in inducing the synthesis of the cellulosome, but non-cellulosic polysaccharides showed no inducing effects.
WOS关键词CRYSTALLINE CELLULOSE ; SIGMA FACTORS ; COMPLEX ; GENE ; POLYSACCHARIDES ; INDUCTION ; PROTEIN ; ULTRASTRUCTURE ; NANOMACHINES ; QUANTITATION
资助项目'Transformational Technologies for Clean Energy and Demonstration', Strategic Priority Research Program of the Chinese Academy of Sciences[XDA 21060201] ; National Key Technology Research and Development Program of China[2015BAD15B05] ; National Natural Science Foundation of China[31570029] ; National Natural Science Foundation of China[31470210] ; National Natural Science Foundation of China[31670735] ; National Natural Science Foundation of China[31661143023] ; Key Technology Research and Development Program of Shandong[2018GSF1160165] ; Qingdao independent innovation major project[16-7-1-11-zdzx-nsh]
WOS研究方向Biotechnology & Applied Microbiology ; Microbiology
语种英语
出版者WILEY
WOS记录号WOS:000442498100010
资助机构'Transformational Technologies for Clean Energy and Demonstration', Strategic Priority Research Program of the Chinese Academy of Sciences ; National Key Technology Research and Development Program of China ; National Natural Science Foundation of China ; Key Technology Research and Development Program of Shandong ; Qingdao independent innovation major project
源URL[http://ir.qibebt.ac.cn/handle/337004/12075]  
专题中国科学院青岛生物能源与过程研究所
通讯作者Cui, Qiu; Liu, Ya-Jun
作者单位1.Chinese Acad Sci, Shandong Prov Key Lab Energy Genet, CAS Key Lab Biofuels, Qingdao, Peoples R China
2.Univ Chinese Acad Sci, Chinese Acad Sci, Beijing, Peoples R China
推荐引用方式
GB/T 7714		 Li, Renmin,Feng, Yingang,Liu, Shiyue,et al. Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum[J]. MICROBIAL BIOTECHNOLOGY,2018,11(5):905-916.
APA Li, Renmin,Feng, Yingang,Liu, Shiyue,Qi, Kuan,Cui, Qiu,&Liu, Ya-Jun.(2018).Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum.MICROBIAL BIOTECHNOLOGY,11(5),905-916.
MLA Li, Renmin,et al."Inducing effects of cellulosic hydrolysate components of lignocellulose on cellulosome synthesis in Clostridium thermocellum".MICROBIAL BIOTECHNOLOGY 11.5(2018):905-916.

入库方式： OAI收割

来源：青岛生物能源与过程研究所

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