Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules
文献类型：期刊论文
| 作者 | Chen, Zhifeng1,2,3; Chen, Jie2,3,4; Ma, Nana2,3,4; Zho, Haifeng1; Cong, Zhiqi2,3 |
| 刊名 | JOURNAL OF PORPHYRINS AND PHTHALOCYANINES
 |
| 出版日期 | 2018-09-01 |
| 卷号 | 22期号:9-10页码:831-836 |
| 关键词 | P450 peroxygenase hydrogen peroxide hydroxylation naphthalene dual-functional small molecules |
| ISSN号 | 1088-4246 |
| DOI | 10.1142/S108842461850061X |
| 英文摘要 | We herein report the H2O2-dependent selective hydroxylation of naphthalene catalyzed by engineered P450BM3 with the assistance of dual-functional small molecules (DFSMs). The mutation at position 268 significantly improved the hydroxylation activity of P450BM3, which is quite different from those engineered P450BM3 peroxygenases and NADPH-dependent P450BM3 mutants previously reported, implicating the unique role of the residue at position 268. This study provides a potential approach to develop the practical hydroxylation biocatalyst of P450s for aromatic hydrocarbons using the DFSM-facilitated P450BM3-H2O2 system. |
| WOS关键词 | CYTOCHROME P450BM-3 ; ACTIVATION ; EVOLUTION ; H2O2 |
| 资助项目 | NSFC[21778060] ; Shandong Provincial Key Laboratory of Synthetic Biology[SPKLSB-2017-02] ; Qingdao Innovative Leading Talent Project |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000442266200017 |
| 出版者 | WORLD SCI PUBL CO INC |
| 资助机构 | NSFC ; Shandong Provincial Key Laboratory of Synthetic Biology ; Qingdao Innovative Leading Talent Project |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/12205]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Zho, Haifeng; Cong, Zhiqi |
| 作者单位 | 1.China Three Gorges Univ, Coll Biol & Pharmaceut Sci, Hubei Key Lab Nat Prod Res & Dev, Yichang 443002, Hubei, Peoples R China 2.Chinese Acad Sci, Shandong Prov Key Lab Synthet Biol, Qingdao 266101, Peoples R China 3.Chinese Acad Sci, Qingdao Inst Bioenergy & Bioproc Technol, CAS Key Lab Biofuels, Qingdao 266101, Peoples R China 4.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chen, Zhifeng,Chen, Jie,Ma, Nana,et al. Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules[J]. JOURNAL OF PORPHYRINS AND PHTHALOCYANINES,2018,22(9-10):831-836. |
| APA | Chen, Zhifeng,Chen, Jie,Ma, Nana,Zho, Haifeng,&Cong, Zhiqi.(2018).Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules.JOURNAL OF PORPHYRINS AND PHTHALOCYANINES,22(9-10),831-836. |
| MLA | Chen, Zhifeng,et al."Selective hydroxylation of naphthalene using the H2O2-dependent engineered P450BM3 driven by dual-functional small molecules".JOURNAL OF PORPHYRINS AND PHTHALOCYANINES 22.9-10(2018):831-836. |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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