Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene
文献类型：期刊论文
| 作者 | Yang,Jianming1; Nie,Qingjuan2; Ren,Meng3; Feng,Hongru4; Jiang,Xinglin1; Zheng,Yanning1; Liu,Min5; Zhang,Haibo1 ; Xian,Mo1
|
| 刊名 | Biotechnology for Biofuels
 |
| 出版日期 | 2013-04-30 |
| 卷号 | 6期号:1 |
| 关键词 | α-Pinene Geranyl diphosphate synthase α-Pinene Synthase E. coli |
| ISSN号 | 1754-6834 |
| DOI | 10.1186/1754-6834-6-60 |
| 英文摘要 | AbstractBackgroundα-Pinene is an important natural product that is widely used in flavorings, fragrances, medicines, fine chemicals and high-density renewable fuels. Currently, α-Pinene used in industry is mainly produced either by tapping trees (gum turpentine) or as a byproduct of paper pulping (crude sulfate turpentine, CST). However, the extraction of it from trees is tedious and inefficient and requires substantial expenditure of natural resources. Therefore, it is necessary to seek sustainable technologies for α-pinene production.ResultsTo construct the microbial synthetic pathway of α-pinene in E. coli, we co-expressed native geranyl diphosphate synthase (IspA) from E. coli and α-pinene synthase (Pt30) from Pinus taeda, and then to increase the geranyl diphosphate (GPP) content in the cells, a suitable geranyl diphosphate synthase (GPPS2) was selected from two different origins. Furthermore, to enhance α-pinene production, a novel biosynthetic pathway of α-pinene was assembled in E. coli BL21(DE3) with the heterologous hybrid mevalonate (MVA) pathway, GPPS2 and α-pinene synthase (Pt30). The final genetic strain, YJM28, harboring the above novel biosynthetic pathway of α-pinene, accumulated α-pinene up to 5.44 mg/L and 0.97 g/L under flask and fed-batch fermentation conditions, respectively. The conversion efficiency of glucose to α-pinene (gram to gram) in the metabolically engineered strain reached 2.61%.ConclusionsIn this paper, by using metabolic engineering techniques, the more efficient biosynthetic pathway of α-pinene was successfully assembled in E. coli BL21(DE3) with the heterologous hybrid MVA pathway, GPPS2 and α-pinene synthase (Pt30). In addition, this is the first report on α-pinene fed-batch fermentation, and our results represent improvements over previous reports. |
| 语种 | 英语 |
| WOS记录号 | BMC:10.1186/1754-6834-6-60 |
| 出版者 | BioMed Central |
| 源URL | [http://ir.qibebt.ac.cn/handle/337004/12586]  |
| 专题 | 中国科学院青岛生物能源与过程研究所 |
| 通讯作者 | Zhang,Haibo; Xian,Mo |
| 作者单位 | 1.Chinese Academy of Sciences; Qingdao Institute of Bioenergy and Bioprocess Technology 2.Qingdao Agricultural University; Foreign Languages School 3.Nanjing Agricultural University; School of Life and Science 4.Shenyang Agricultural University; College of Bioscience and Biotechnology 5.Wuhan University of Science and Technology; College of Chemical Engineering and Technology |
| 推荐引用方式 GB/T 7714 | Yang,Jianming,Nie,Qingjuan,Ren,Meng,et al. Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene[J]. Biotechnology for Biofuels,2013,6(1). |
| APA | Yang,Jianming.,Nie,Qingjuan.,Ren,Meng.,Feng,Hongru.,Jiang,Xinglin.,...&Xian,Mo.(2013).Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene.Biotechnology for Biofuels,6(1). |
| MLA | Yang,Jianming,et al."Metabolic engineering of Escherichia colifor the biosynthesis of alpha-pinene".Biotechnology for Biofuels 6.1(2013). |
入库方式： OAI收割
来源：青岛生物能源与过程研究所
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