Incorporation of Cerium Oxide into Hydroxyapatite Coating Protects Bone Marrow Stromal Cells Against H2O2-Induced Inhibition of Osteogenic Differentiation
文献类型：期刊论文
| 作者 | Li, Kai; Shen, Qingyi1; Xie, Youtao; You, Mingyu; Huang, Liping; Zheng, Xuebin |
| 刊名 | BIOLOGICAL TRACE ELEMENT RESEARCH
 |
| 出版日期 | 2018 |
| 卷号 | 182期号:1页码:91 |
| 关键词 | Oxidative stress Osteogenic differentiation CeO2 Hydroxyapatite Antioxidant |
| ISSN号 | 0163-4984 |
| DOI | 10.1007/s12011-017-1066-3 |
| 英文摘要 | Oxidative stress exerts a key influence in osteoporosis in part by inhibiting osteogenic differentiation of bone marrow stromal cells (BMSCs). With their unique antioxidant properties and reported biocompatibility, cerium oxide (CeO2) ceramics exhibit promising potential for the treatment of osteoporosis resulting from oxidative stress. In this study, protective effects of CeO2-incorporated hydroxyapatite coatings (HA-10Ce and HA-30Ce) on the viability and osteogenic differentiation of H2O2-treated BMSCs were examined. CeO2-incorporated HA coatings enhanced cell viability and attenuated cell apoptosis caused by H2O2. An increase in CeO2 content in HA coatings better alleviated H2O2-induced inhibition of osteogenic differentiation by increasing alkaline phosphatase (ALP) activity, calcium deposition activity, and mRNA expression levels of osteogenesis markers runt-related transcription factor 2 (Runx2), ALP, and osteocalcin (OCN) in BMSCs. Furthermore, the H2O2-induced decrease of gene and protein expressions of beta-catenin and cyclin D1 in the Wnt/beta-catenin signaling pathway was successfully rescued by the CeO2 incorporated HA coatings. Besides, the decreased expression of receptor activator of nuclear factor kappa-B ligand (RANKL) and the increased ratio of osteoprotegerin (OPG)/RANKL in BMSCs on the CeO2-modified coatings was observed, indicating the inhibition of osteoclastogenesis. The above results were mediated by the antioxidant properties of CeO2. The CeO2-incorporated HA coatings reversed the decreased superoxide dismutase (SOD) activity, reduced reactive oxygen species (ROS) generation, and suppressed the malondiadehyde (MDA) formation. The findings suggested that CeO2-modified HA coatings may be promising coating materials for osteoporotic bone regeneration. |
| 学科主题 | Biochemistry & Molecular Biology ; Endocrinology & Metabolism |
| WOS记录号 | WOS:000424700000010 |
| 出版者 | HUMANA PRESS INC |
| 资助机构 | This work was supported by the Natural Science Foundation of China (Grant No. 51502328) and the Opening Project of the Shanghai Key Laboratory of Orthopedic Implant (Grant No. KFKT2016003). ; This work was supported by the Natural Science Foundation of China (Grant No. 51502328) and the Opening Project of the Shanghai Key Laboratory of Orthopedic Implant (Grant No. KFKT2016003). |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24451]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Ceram, Key Lab Inorgan Coating Mat, 1295 Dingxi Rd, Shanghai 200050, Peoples R China 2.Shanghai Stomatol Dis Ctr, Dept Prosthodont, Shanghai 200031, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Kai,Shen, Qingyi,Xie, Youtao,et al. Incorporation of Cerium Oxide into Hydroxyapatite Coating Protects Bone Marrow Stromal Cells Against H2O2-Induced Inhibition of Osteogenic Differentiation[J]. BIOLOGICAL TRACE ELEMENT RESEARCH,2018,182(1):91, 104. |
| APA | Li, Kai,Shen, Qingyi,Xie, Youtao,You, Mingyu,Huang, Liping,&Zheng, Xuebin.(2018).Incorporation of Cerium Oxide into Hydroxyapatite Coating Protects Bone Marrow Stromal Cells Against H2O2-Induced Inhibition of Osteogenic Differentiation.BIOLOGICAL TRACE ELEMENT RESEARCH,182(1),91. |
| MLA | Li, Kai,et al."Incorporation of Cerium Oxide into Hydroxyapatite Coating Protects Bone Marrow Stromal Cells Against H2O2-Induced Inhibition of Osteogenic Differentiation".BIOLOGICAL TRACE ELEMENT RESEARCH 182.1(2018):91. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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