Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber
文献类型：期刊论文
| 作者 | Li, Xiuchun; Yan, Shanshan1; Dai, Jing1; Lu, Yi; Wang, Yiqun2; Sun, Man3; Gong, Jinkang; Yao, Yuan |
| 刊名 | COLLOIDS AND SURFACES B-BIOINTERFACES
 |
| 出版日期 | 2018 |
| 卷号 | 162页码:390 |
| 关键词 | Electrospinning Nanofiber PVA/Collagen A549 cell Epithelial-mesenchymal transition |
| ISSN号 | 0927-7765 |
| DOI | 10.1016/j.colsurfb.2017.12.010 |
| 英文摘要 | Epithelial-mesenchymal transition (EMT) is a process by which epithelial cells lose their cell cell contact to become mesenchymal stem cells, which is important on development and embryogenesis, wound healing, and cancer metastasis. This research aims to investigate the effect of topological cue as modulating factor on the EMT by tuning the diameter of electrospinning nanofiber. The cell-nanofiber interaction between human lung epithelial cell A549 and electrospinning nanofibers composed of polyvinyl alcohol (PVA) and type I collagen were investigated. The electrospinning of regenerated PVA/Collagen nanofibers were performed with water/acetic acid as a spinning solvent and glutaraldehyde as a chemical cross linker. Parameterization on concentration, applied voltage and feeding rate was finalized to generate smooth nanofibers with good homogeneity. The scanning electron microscopy result demonstrated that A549 cell appropriately achieved extended morphology by the filopodia attaching to the surface of the nanofibrous mats. When the diameter changed from 90 nm to 240 nm, the A549 cell was correspondingly express varied EMT related genes. Gene expression analysis was conducted by qPCR using three typical markers for detecting EMT: N-cadherin (NCad), Vimentin (Vim), and Fibronectin (Fib). An increasing expression pattern was observed on cell culturing on 170 nm sample with respect to cell cultured on 90 nm and 240 nm. This result indicated the 170 nm PVA/Collagen nanofibers induce A549 cells to process epithelial mesenchymal transition more seriously than those on 90 nm or 240 nm. (C) 2017 Elsevier B.V. All rights reserved. |
| 学科主题 | Biophysics ; Chemistry, Physical ; Materials Science, bioMaterials |
| WOS记录号 | WOS:000425076500046 |
| 出版者 | ELSEVIER SCIENCE BV |
| 资助机构 | This work was funded by ShanghaiTech University. Authors would like to appreciate Dr Yanyan Jia, Shanghaitech University for the guidance of the scanning electron microscopy. ; This work was funded by ShanghaiTech University. Authors would like to appreciate Dr Yanyan Jia, Shanghaitech University for the guidance of the scanning electron microscopy. |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24513]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.ShanghaiTech Univ, Sch Phys Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, 1295 Dingxi Rd, Shanghai 200050, Peoples R China 3.Chinese Acad Sci, Shanghai Inst Organ Chem, 345 Lingling Lu, Shanghai 200032, Peoples R China 4.ShanghaiTech Univ, Sch Life Sci & Technol, 393 Middle Huaxia Rd, Shanghai 201210, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Xiuchun,Yan, Shanshan,Dai, Jing,et al. Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber[J]. COLLOIDS AND SURFACES B-BIOINTERFACES,2018,162:390, 397. |
| APA | Li, Xiuchun.,Yan, Shanshan.,Dai, Jing.,Lu, Yi.,Wang, Yiqun.,...&Yao, Yuan.(2018).Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber.COLLOIDS AND SURFACES B-BIOINTERFACES,162,390. |
| MLA | Li, Xiuchun,et al."Human lung epithelial cells A549 epithelial-mesenchymal transition induced by PVA/Collagen nanofiber".COLLOIDS AND SURFACES B-BIOINTERFACES 162(2018):390. |
入库方式： OAI收割
来源：上海硅酸盐研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。