In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes
文献类型：期刊论文
| 作者 | Zhang, Hua; Wu, Yue; Wang, Jing; Tang, Zhongmin1; Ren, Yan; Ni, Dalong1; Gao, Hongbo2; Song, Ruixue3; Jin, Teng; Li, Qiao |
| 刊名 | SMALL
 |
| 出版日期 | 2018 |
| 卷号 | 14期号:3 |
| ISSN号 | 1613-6810 |
| 关键词 | adoptive T-Cells glioma magnetic resonance imaging NaGdF4 TAT |
| DOI | 10.1002/smll.201702951 |
| 英文摘要 | Adoptive T lymphocyte immunotherapy is one of the most promising methods to treat residual lesions after glioma surgery. However, the fate of the adoptively transferred T-cells in vivo is unclear, hampering the understanding of this emerging therapy. Thus, it is highly desirable to develop noninvasive and quantitative in vivo tracking of these T-cells to glioma for better identification of the migratory fate and to provide objective evaluation of outcomes of adoptive T-cell immunotherapy targeting glioma. In this work, ultrasmall T-1 MR-based nanoprobes, NaGdF4-TAT, as molecular probes with high longitudinal relaxivity (8.93 mm(-1) s(-1)) are designed. By means of HIV-1 transactivator (TAT) peptides, nearly 95% of the adoptive T-cells are labeled with the NaGdF4-TAT nanoprobes without any measurable side effects on the labeled T-cells, which is remarkably superior to that of the control fluorescein isothiocyanate-NaGdF4 concerning labeling efficacy. Labeled adoptive T-cell clusters can be sensitively tracked in an orthotopic GL261-glioma model 24 h after intravenous infusion of 10(7) labeled T-cells by T-1-weighted MR imaging. Both in vitro and in vivo experiments show that the NaGdF4-TAT nanoprobes labeling of T-cells may be a promising method to track adoptive T-cells to improve our understanding of the pathophysiology in adoptive immunotherapy for gliomas. |
| 学科主题 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| 出版者 | WILEY-V C H VERLAG GMBH |
| WOS记录号 | WOS:000422789800012 |
| 资助机构 | H.Z. and Y.W. contributed equally this work. W.B. and Z.Y. are the corresponding authors. The authors thank Chen Zhang, Yanyan Liu, and Han Wang from Shanghai Institute of Ceramics Chinese Academy of Sciences for TEM and optical analysis. Meanwhile, the authors also thank Shuying Chen from Laboratory Medicine, Huashan Hospital for analysis of the flow cytometry results. This work was financially supported by the National Natural Science Foundation of China Research (Grant Nos. 81671732 and 51372260), the China National Funds for Distinguished Young Scientists (Grant No. 51725202), and the Shanghai Excellent Academic Leaders Program (Grant No. 16XD1404000). ; H.Z. and Y.W. contributed equally this work. W.B. and Z.Y. are the corresponding authors. The authors thank Chen Zhang, Yanyan Liu, and Han Wang from Shanghai Institute of Ceramics Chinese Academy of Sciences for TEM and optical analysis. Meanwhile, the authors also thank Shuying Chen from Laboratory Medicine, Huashan Hospital for analysis of the flow cytometry results. This work was financially supported by the National Natural Science Foundation of China Research (Grant Nos. 81671732 and 51372260), the China National Funds for Distinguished Young Scientists (Grant No. 51725202), and the Shanghai Excellent Academic Leaders Program (Grant No. 16XD1404000). |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24546]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.Fudan Univ, Huashan Hosp, Dept Radiol, Shanghai 200040, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 3.Fudan Univ, Shanghai Canc Ctr, Dept Radiol, Shanghai 200032, Peoples R China 4.East China Normal Univ, Sch Chem & Mol Engn, Shanghai Key Lab Green Chem & Chem Proc, Shanghai 200062, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Hua,Wu, Yue,Wang, Jing,et al. In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes[J]. SMALL,2018,14(3). |
| APA | Zhang, Hua.,Wu, Yue.,Wang, Jing.,Tang, Zhongmin.,Ren, Yan.,...&Yao, Zhenwei.(2018).In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes.SMALL,14(3). |
| MLA | Zhang, Hua,et al."In Vivo MR Imaging of Glioma Recruitment of Adoptive T-Cells Labeled with NaGdF4-TAT Nanoprobes".SMALL 14.3(2018). |
入库方式： OAI收割
来源：上海硅酸盐研究所
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