Selective non-enzymatic total bilirubin detection in serum using europium complexes with different beta-diketone-derived ligands as luminescence probes
文献类型：期刊论文
| 作者 | Yang, Wei; Xia, Jinfeng1; Zhou, Guohong1; Jiang, Danyu1; Li, Qiang; Wang, Shiwei1; Zheng, Xiaohong; Li, Xi; Shen, Yibo; Li, Xin |
| 刊名 | ANALYTICAL AND BIOANALYTICAL CHEMISTRY
 |
| 出版日期 | 2018 |
| 卷号 | 410期号:25页码:6459 |
| 关键词 | Total bilirubin Europium(III) complex Biosensor Fluorescence quenching sigma-hole |
| ISSN号 | 1618-2642 |
| DOI | 10.1007/s00216-018-1243-z |
| 英文摘要 | Three europium(III) complexes, Eu(ectfd)(3) (Hectfd = 1-(9-ethyl-9H-carbazol-7-yl)-4,4,4-trifluorobutane-1,3-dione), Eu(tta)(3) (Htta = 4,4,4-trifluoro-1-(thiophen-2-yl)-butane-1,3-dione), and Eu(dbt)(3) (Hdbt = 2-(4',4',4'-trifluoro-1',3'-dioxobutyl)dibenzothiophene), were synthesized and employed to detect total bilirubin (BR) in blood-serum samples. UV-visible absorption and fluorescence (FL) spectroscopies were used to evaluate the selectivity of each europium (III) fluorescence probe to BR, which was shown to remarkably reduce the luminescence intensities of the europium(III) complexes at a wavelength of 612 nm. The luminescence intensity of each complex is linearly related to BR concentration. Eu(tta)(3) was shown to be the more-appropriate fluorescence probe for the sensitive and reliable detection of total BR in blood serum samples than either Eu(ectfd)(3) or Eu(dbt)(3). This observation can be ascribed to special sigma-hole bonding between Htta and BR. In addition, the optimal pH test conditions for the detection of BR in human serum by the Eu(tta)(3) probe were determined. Sensitivity was shown to be dramatically affected by the pH of the medium. The experimental results reveal that pH 7.5 is optimal for this probe, which coincides with the pH of human serum. Furthermore, BR detection using the Eu(tta)(3) luminescence probe is simple, practical, and relatively free of interference from coexisting substances; it has a minimum detection limit (DL) of 68 nM and is a potential candidate for the routine assessment of total BR in serum samples. |
| 学科主题 | Biochemical Research Methods ; Chemistry, Analytical |
| WOS记录号 | WOS:000444158500010 |
| 出版者 | SPRINGER HEIDELBERG |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24647]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.East China Normal Univ, Dept Chem, Shanghai 200062, Peoples R China 2.Chinese Acad Sci, Shanghai Inst Ceram, Shanghai 200050, Peoples R China |
| 推荐引用方式 GB/T 7714 | Yang, Wei,Xia, Jinfeng,Zhou, Guohong,et al. Selective non-enzymatic total bilirubin detection in serum using europium complexes with different beta-diketone-derived ligands as luminescence probes[J]. ANALYTICAL AND BIOANALYTICAL CHEMISTRY,2018,410(25):6459, 6468. |
| APA | Yang, Wei.,Xia, Jinfeng.,Zhou, Guohong.,Jiang, Danyu.,Li, Qiang.,...&Li, Xin.(2018).Selective non-enzymatic total bilirubin detection in serum using europium complexes with different beta-diketone-derived ligands as luminescence probes.ANALYTICAL AND BIOANALYTICAL CHEMISTRY,410(25),6459. |
| MLA | Yang, Wei,et al."Selective non-enzymatic total bilirubin detection in serum using europium complexes with different beta-diketone-derived ligands as luminescence probes".ANALYTICAL AND BIOANALYTICAL CHEMISTRY 410.25(2018):6459. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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