Fe-Au Nanoparticle-Coupling for Ultrasensitive Detections of Circulating Tumor DNA
文献类型：期刊论文
| 作者 | Hu, Ping; Zhang, Shengjian1; Wu, Tong2; Ni, Dalong; Fan, Wenpei; Zhu, Yan; Qian, Rong; Shi, Jianlin |
| 刊名 | ADVANCED MATERIALS
 |
| 出版日期 | 2018 |
| 卷号 | 30期号:31 |
| 关键词 | amorphous iron circulating tumor DNA early diagnosis liquid biopsy nanoparticle-coupling strategy |
| ISSN号 | 0935-9648 |
| DOI | 10.1002/adma.201801690 |
| 英文摘要 | Effectiveness of cancer therapy relies heavily on the efficient early diagnosis. Circulating tumor DNA (ctDNA) detection is one of the most clinically meaningful liquid biopsy approaches for the noninvasive cancer early diagnosis, which, unfortunately, cannot be applied as a routine diagnostic tool till a number of obstacles, for example, unsatisfactory specificity and sensitivity, and extremely high costs, are overcome. Here, the first paradigm of nanomaterial's application in the extremely specific, ultrasensitive, and yet economical ctDNA detections is reported based on a facile nanoparticle-coupling strategy without amplification, with which polymerase chain reaction (PCR)-introduced bias and other shortcomings are successfully circumvented. Aiming at seven Kirsten rat sarcoma-2 virus (KRAS) point mutations, the present strategy exhibits high specificity and an ultrahigh sensitivity of detecting as low as 0.1 pg mL(-1) of KRAS point mutation without prior PCR amplification. Discriminating KRAS gene mutations in lung adenocarcinoma patients at an extremely low detection limit equivalent to 0.12% mutation relative to wild-type gene is successful. It is envisioned that this nanoparticle-coupling approach could be routinely applied clinically for ultra-early diagnosis and monitoring of diverse malignant tumors, thus facilitating the fight against cancer. |
| 学科主题 | Chemistry, Multidisciplinary ; Chemistry, Physical ; Nanoscience & Nanotechnology ; Materials Science, Multidisciplinary ; Physics, Applied ; Physics, Condensed Matter |
| WOS记录号 | WOS:000443807400011 |
| 出版者 | WILEY-V C H VERLAG GMBH |
| 资助机构 | P.H. and S.Z. contributed equally to this work. This work was financially supported by the National Natural Science Foundation of China (Grant No. 51702349), Shanghai Yangfan Program (16YF1412800), and Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures (SKL201704). The authors thank Jingwei Feng, Heliang Yao, and Linlin Zhang from Shanghai Institute of Ceramics, Chinese Academy of Sciences, for TEM measurement and structure analysis. Animal experimental procedures were in agreement with the guidelines of the Regional Ethics Committee for Animal Experiments and the care regulations approved by the administrative committee of laboratory animals of Fudan University. Routine blood collection and tumor tissue determination of patients were conducted in clinical center. Written informed consent was obtained from all participants before the onset of study. This study was approved by the medical ethics committee of Fudan University Shanghai Cancer Center (IRB#090977-1). ; P.H. and S.Z. contributed equally to this work. This work was financially supported by the National Natural Science Foundation of China (Grant No. 51702349), Shanghai Yangfan Program (16YF1412800), and Science Foundation for Youth Scholar of State Key Laboratory of High Performance Ceramics and Superfine Microstructures (SKL201704). The authors thank Jingwei Feng, Heliang Yao, and Linlin Zhang from Shanghai Institute of Ceramics, Chinese Academy of Sciences, for TEM measurement and structure analysis. Animal experimental procedures were in agreement with the guidelines of the Regional Ethics Committee for Animal Experiments and the care regulations approved by the administrative committee of laboratory animals of Fudan University. Routine blood collection and tumor tissue determination of patients were conducted in clinical center. Written informed consent was obtained from all participants before the onset of study. This study was approved by the medical ethics committee of Fudan University Shanghai Cancer Center (IRB#090977-1). |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24744]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 2.Fudan Univ, Shanghai Med Coll, Canc Hosp, Inst & Dept Oncol,Dept Radiol, Shanghai 200032, Peoples R China 3.Stanford Univ, Dept Mat Sci & Engn, Stanford, CA 94305 USA |
| 推荐引用方式 GB/T 7714 | Hu, Ping,Zhang, Shengjian,Wu, Tong,et al. Fe-Au Nanoparticle-Coupling for Ultrasensitive Detections of Circulating Tumor DNA[J]. ADVANCED MATERIALS,2018,30(31). |
| APA | Hu, Ping.,Zhang, Shengjian.,Wu, Tong.,Ni, Dalong.,Fan, Wenpei.,...&Shi, Jianlin.(2018).Fe-Au Nanoparticle-Coupling for Ultrasensitive Detections of Circulating Tumor DNA.ADVANCED MATERIALS,30(31). |
| MLA | Hu, Ping,et al."Fe-Au Nanoparticle-Coupling for Ultrasensitive Detections of Circulating Tumor DNA".ADVANCED MATERIALS 30.31(2018). |
入库方式： OAI收割
来源：上海硅酸盐研究所
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