The role of the micro-pattern and nano-topography of hydroxyapatite bioceramics on stimulating osteogenic differentiation of mesenchymal stem cells
文献类型：期刊论文
| 作者 | Zhao, Cancan1; Wang, Xiaoya; Gao, Long1; Jing, Linguo; Zhou, Quan; Chang, Jiang |
| 刊名 | ACTA BIOMATERIALIA
 |
| 出版日期 | 2018 |
| 卷号 | 73页码:509 |
| 关键词 | Micro/nano hybrid Osteogenic differentiation Synergistic effect Integrins BMP2 signaling pathway Cell-cell communication |
| ISSN号 | 1742-7061 |
| DOI | 10.1016/j.actbio.2018.04.030 |
| 英文摘要 | The micro/nano hybrid structure is considered to be a biomaterial characteristic to stimulate osteogenesis by mimicking the three-dimensional structure of the bone matrix. However, the mechanism of the hybrid structure induced osteogenic differentiation of stem cells is still unknown. For elucidating the mechanisms, one of the challenge is to directly fabricate micro/nano hybrid structure on bioceramics because of its brittleness. In this study, hydroxyapatite (HA) bioceramics with the micro/nano hybrid structure were firstly fabricated via a hydrothermal treatment and template method, and the effect of the different surface structures on the expression of integrins, BMP2 signaling pathways and cell-cell communication was investigated. Interestingly, the results suggested that the osteogenic differentiation induced by micro/nano structures was modulated first through activating integrins and then further activating BMP2 signaling pathway and cell-cell communication, while activated BMP2 could in turn activate integrins and Cx43-related cell cell communication. Furthermore, differences in activation of integrins, BMP2 signaling pathway, and gap junction-mediated cell-cell communication were observed, in which nanorod and micropattern structures activated different integrin subunits, BMP downstream receptors and Cx43. This finding may explain the synergistic effect of the micro/nano hybrid structure on the activation of osteogenic differentiation of BMSCs. Based on our study, we concluded that the different activation mechanisms of micro- and nano structures led to the synergistic stimulatory effect on integrin activation and osteogenesis, in which not only the direct contact of cells on micro/nano structure played an important role, but also other surface characteristics such as protein adsorption might contribute to the bioactive effect. Statement of Significance The micro/nano hybrid structure has been found to have synergistic bioactivity on osteogenesis. However, it is still a challenge to fabricate the hybrid structure directly on the bioceramics, and the role of micro- and nano-structure, in particular the mechanism of the micro/nano-hybrid structure induced stem cell differentiation is still unknown. In this study, we firstly fabricated hydroxyapatite bioceramics with the micro/nano hybrid structure, and then investigated the effect of different surface structure on expression of integrins, BMP2 signaling pathways and cell-cell communication. Interestingly, we found that the osteogenic differentiation induced by structure was modulated first through activating integrins and then further activating BMP2 signaling pathway and cell-cell communication, and activated BMP2 could in turn activate some integrin subunits and Cx43-related cell-cell communication. Furthermore, differences in activation of integrins, BMP2 signaling pathway, and gap junction-mediated cell-cell communication were observed, in which nanorod and micropattern structures activated different integrin subunits, BMP downstream receptors and Cx43. This finding may explain the synergistic effect of the micro/nano hybrid structure on the activation of osteogenic differentiation of BMSCs. Based on our study, we concluded that the different activation mechanisms of micro- and nano-structures led to the synergistic stimulatory effect on integrin activation and osteogenesis, in which not only the direct contact of cells on micro/nano structure played an important role, but also other surface characteristics such as protein adsorption might contribute to the bioactive effect. (C) 2018 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved. |
| 学科主题 | Engineering, Biomedical ; Materials Science, bioMaterials |
| WOS记录号 | WOS:000436222600041 |
| 出版者 | ELSEVIER SCI LTD |
| 资助机构 | Funding for this study was provided by the National Natural Science Foundation of China (Grant No. 81430012), and the International S&T Cooperation Project of Shanghai S&T Innovation Action Plan (Grant No.:17540712300). ; Funding for this study was provided by the National Natural Science Foundation of China (Grant No. 81430012), and the International S&T Cooperation Project of Shanghai S&T Innovation Action Plan (Grant No.:17540712300). |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24879]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhao, Cancan,Wang, Xiaoya,Gao, Long,et al. The role of the micro-pattern and nano-topography of hydroxyapatite bioceramics on stimulating osteogenic differentiation of mesenchymal stem cells[J]. ACTA BIOMATERIALIA,2018,73:509, +. |
| APA | Zhao, Cancan,Wang, Xiaoya,Gao, Long,Jing, Linguo,Zhou, Quan,&Chang, Jiang.(2018).The role of the micro-pattern and nano-topography of hydroxyapatite bioceramics on stimulating osteogenic differentiation of mesenchymal stem cells.ACTA BIOMATERIALIA,73,509. |
| MLA | Zhao, Cancan,et al."The role of the micro-pattern and nano-topography of hydroxyapatite bioceramics on stimulating osteogenic differentiation of mesenchymal stem cells".ACTA BIOMATERIALIA 73(2018):509. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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