Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis
文献类型：期刊论文
| 作者 | Zhang, Xihui4; Yang, Liecheng4; Liu, Yongming4; Song, Zhentao3; Zhao, Jian3; Chen, Dandan; Yu, Huan; Li, Ruibin; Wang, Yangyun; Yang, Kai |
| 刊名 | TOXICOLOGY AND APPLIED PHARMACOLOGY
 |
| 出版日期 | 2018 |
| 卷号 | 348页码:54 |
| 关键词 | Phospholipidosis Nanocarrier Primary hepatocyte Spheroid High content screening |
| ISSN号 | 0041-008X |
| DOI | 10.1016/j.taap.2018.04.016 |
| 英文摘要 | Considerable effort has been made to develop nanocarriers for controlled drug delivery over the last decade, while it remains unclear how the strength of adverse drug effect will be altered when a drug is loaded on the nanocarrier. Drug-induced phospholipidosis (DIP) is characterized with excessive accumulation of phospholipids in cells and is common for cationic amphiphilic drugs (CAD). Previously, we have reported that PEGylated graphene oxide (PEG-GO) loaded with several CAD can potentiate DIP. In current study, we extended our study on newly identified phospholipidosis (PLD) inducers that had been identified from the Library of Pharmacologically Active Compounds (LOPAC), to investigate if PEO-GO loaded with these CAD can alter DIP. Twenty-two CAD were respectively loaded on PEG-GO and incubated with RAW264.7, a macrophage cell line. The results showed that when a CAD was loaded on PEG-GO, its strength of PLD induction can be enhanced, unchanged or attenuated. PEG-GO loaded with Ifenprodil exhibited the highest PEG-GO potentiation effect compared to Ifenprodil treatment alone in RAW264.7 cells, and this effect was confirmed in human hepatocellular carcinoma HepG2, another cell line model for PLD induction. Primary hepatocyte culture and spheroids mimicking in vivo conditions were used to further validate nanocarrier potentiation on DIP by Ifenprodil. Stronger phospholipid accumulation was found in PEG-GO/Ifenprodil treated hepatocytes or spheroids than Ifenprodil treatment alone. Therefore, evidences were provided by us that nanocarriers may increase the adverse drug effects and guidance by regulatory agencies need to be drafted for the safe use of nanotechnology in drug delivery. |
| 学科主题 | Pharmacology & Pharmacy ; Toxicology |
| WOS记录号 | WOS:000432634300006 |
| 出版者 | ACADEMIC PRESS INC ELSEVIER SCIENCE |
| 资助机构 | The authors would like to thank the National Natural Science Foundation of China (#81401511, #31771104, #31400861, #11304212), Key Project of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (17KJA310003), Natural Science Foundation of Jiangsu Province (BK20130279), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection for financial support. ; The authors would like to thank the National Natural Science Foundation of China (#81401511, #31771104, #31400861, #11304212), Key Project of Natural Science Foundation of the Higher Education Institutions of Jiangsu Province (17KJA310003), Natural Science Foundation of Jiangsu Province (BK20130279), Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD), and Jiangsu Provincial Key Laboratory of Radiation Medicine and Protection for financial support. |
| 源URL | [http://ir.sic.ac.cn/handle/331005/24893]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.[Zhang, Xihui; Yang, Liecheng; Liu, Yongming; Chen, Dandan; Yu, Huan; Li, Ruibin; Wang, Yangyun; Yang, Kai; Zhang, Leshuai W.] Soochow Univ, Collaborat Innovat Ctr Radiat Med Jiangsu Higher, Sch Radiol & Interdisciplinary Sci RAD X, State Key Lab Radiat Med & Protect,Sch Radiat Med, Suzhou 215123, Peoples R China 2.NIH, Natl Ctr Advancing Translat Sci, Bldg 10, Bethesda, MD 20892 USA 3.Chinese Acad Sci, Shanghai Inst Ceram, State Key Lab High Performance Ceram & Superfine, Shanghai 200050, Peoples R China 4.East China Univ Sci & Technol, State Key Lab Bioreactor Engn, Shanghai 200237, Peoples R China 5.Soochow Univ, Sch Biol & Basic Med Sci, Suzhou 215123, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, Xihui,Yang, Liecheng,Liu, Yongming,et al. Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis[J]. TOXICOLOGY AND APPLIED PHARMACOLOGY,2018,348:54, 66. |
| APA | Zhang, Xihui.,Yang, Liecheng.,Liu, Yongming.,Song, Zhentao.,Zhao, Jian.,...&Zhang, Leshuai W..(2018).Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis.TOXICOLOGY AND APPLIED PHARMACOLOGY,348,54. |
| MLA | Zhang, Xihui,et al."Detection of nanocarrier potentiation on drug induced phospholipidosis in cultured cells and primary hepatocyte spheroids by high content imaging and analysis".TOXICOLOGY AND APPLIED PHARMACOLOGY 348(2018):54. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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