Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo
文献类型：期刊论文
| 作者 | Fan, Qingfei; Liu, Yang; Kulakowski, Daniel; Chen, Shaonong; Friesen, J. Brent; Pauli, Guido F.; Song, Qishi
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| 刊名 | JOURNAL OF CHROMATOGRAPHY A
 |
| 出版日期 | 2018 |
| 卷号 | 1553期号:x页码:108-115 |
| 关键词 | Countercurrent Separation Mammalian Steroid Hormones Progesterone Estriol Guess Method |
| ISSN号 | 0021-9673 |
| 英文摘要 | Countercurrent separation (CCS) has been widely used for the separation of high abundance compounds. However, the identification of low abundance compounds, such as mammalian steroid hormones, from natural sources is still a challenging task. A mixture of 14 human steroid hormone reference compounds was prepared for the development of a CCS enrichment strategy. The TLC-based GUESS (Generally Useful Estimate of Solvent Systems) method along with partitioning experiments were implemented to develop a process for the enrichment of these low abundance compounds with CCS. The application of CCS to the steroid hormone enrichment of Vitex negundo extracts was demonstrated by the identification of progesterone and estriol. This method provides a CCS-driven strategy to mine plant sources for low abundance compounds. (C) 2018 Elsevier B.V. All rights reserved. |
| URL标识 | 查看原文 |
| 语种 | 英语 |
| 公开日期 | 2018-06-07 |
| 源URL | [http://ir.xtbg.org.cn/handle/353005/10856]  |
| 专题 | 西双版纳热带植物园_民族植物学研究组 中国科学院西双版纳热带植物园 |
| 推荐引用方式 GB/T 7714 | Fan, Qingfei,Liu, Yang,Kulakowski, Daniel,et al. Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo[J]. JOURNAL OF CHROMATOGRAPHY A,2018,1553(x):108-115. |
| APA | Fan, Qingfei.,Liu, Yang.,Kulakowski, Daniel.,Chen, Shaonong.,Friesen, J. Brent.,...&Song, Qishi.(2018).Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo.JOURNAL OF CHROMATOGRAPHY A,1553(x),108-115. |
| MLA | Fan, Qingfei,et al."Countercurrent separation assisted identification of two mammalian steroid hormones in Vitex negundo".JOURNAL OF CHROMATOGRAPHY A 1553.x(2018):108-115. |
入库方式： OAI收割
来源：西双版纳热带植物园
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