Plants are moving poleward and upward in response to climate warming. However, such movements lag behind the expanding warming front for many reasons, including the impediment of plant movement caused by unusual cold events. In this study, we measured the maximum photochemical efficiency of photosystem II (F-v/F-m) in 101 warm-climate angiosperm species to assess their cold tolerance at the end of a severe chilling period of 49 days in a southern subtropical region (Nanning) in China. We found that 36 of the 101 species suffered from chilling-induced physiological injury, with predawn F-v/F-m values of less than 0.7. There was a significant exponential relationship between the predawn F-v/F-m and northern latitudinal limit of a species; species with a lower latitudinal limit suffered more. Our results suggest that the range limits of warm-climate plants are potentially influenced by their physiological sensitivity to chilling temperatures and that their poleward movement might be impeded by extreme cold events. The quick measurement of F-v/F-m is useful for assessing the cold tolerance of plants, providing valuable information for modelling species range shifts under changing climate conditions and species selection for horticultural management and urban landscape design.