Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis
文献类型：期刊论文
| 作者 | Li, WJ; Zhong, Y; Lin, BC; Su, ZG |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2001-01-05 |
| 卷号 | 905期号:1-2页码:299-307 |
| 关键词 | semi-aqueous capillary electrophoresis capillary electrophoresis buffer composition proteins poly(ethylene glycol) |
| 英文摘要 | a new program to characterize polyethylene glycol-modified (pegylated) proteins is outlined using capillary zone electrophoresis (cze). pegylated ribonuclease a and lysozyme were selected as examples. five separation procedures were compared to select out the mixed buffer of acetonitrile-water (1:1, v/v) at ph 2.5 as the best to characterize the pegylated proteins without sample pretreatment. polyethylene oxide (peo) with a high molecular mass of 8x10(6) was applied to rinse the capillary to form a dynamic coating which would decrease the undesirable proteins adsorbed to the inner wall of the silica. the electroosmotic flow (eof) mobility of the five procedures was determined, respectively. it is found that acetonitrile is mainly responsible for the good resolution of pegylated proteins with the help of peo coating in the semi-aqueous system. the low eof mobility and current in the semi-aqueous system might also have some responsibility for the high resolution. the semi-aqueous procedure described in this paper also demonstrates higher resolution of natural proteins than aqueous ones. (c) 2001 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | modified superoxide-dismutase ; modified lysozyme ; asparaginase ; ribonuclease ; activation ; enzymes ; charge |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000166339600029 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84611]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, State Key Lab Biochem Engn, Beijing 100080, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, WJ,Zhong, Y,Lin, BC,et al. Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis[J]. journal of chromatography a,2001,905(1-2):299-307. |
| APA | Li, WJ,Zhong, Y,Lin, BC,&Su, ZG.(2001).Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis.journal of chromatography a,905(1-2),299-307. |
| MLA | Li, WJ,et al."Characterization of polyethylene glycol-modified proteins by semi-aqueous capillary electrophoresis".journal of chromatography a 905.1-2(2001):299-307. |
入库方式： OAI收割
来源：大连化学物理研究所
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