Total analytical method for Radix astragali extract using two-binary multi-segment gradient elution liquid chromatography
文献类型：期刊论文
| 作者 | Xiao, HB; Liang, XM; Lu, PC |
| 刊名 | journal of separation science
 |
| 出版日期 | 2001-03-01 |
| 卷号 | 24期号:3页码:186-196 |
| 关键词 | column liquid chromatography method development computer simulation gradient optimization Radix astragali complex sample |
| 英文摘要 | there is an urgent need for thorough analysis of radix astragali, a widely used chinese herb, for quality control purposes. this paper describes the development of a total analytical method for radix astragali extract, a multi-component complex mixture. twenty-four components were separated step by step from the extract using a series of isocratic isopropanol-methanol elutions, and then 42 components were separated similarly using methanol-water elutions. based on the log k(w) and -s of the 66 components obtained from the above procedure and the optimization software developed in our laboratory, an optimum elution program consisting of seven methanol-water segments and four isopropanol-methanol segments was developed to finish the task of analyzing the total components in a single run. under optimized gradient conditions, the sample of radix astragali extract was analyzed. as expected, most of the components were well separated and the experimental chromatogram was in a good agreement with the predicted one. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | optimizing temperature ; complex samples ; optimization ; separation ; constituents ; tang |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000167947800005 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84617]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xiao, HB,Liang, XM,Lu, PC. Total analytical method for Radix astragali extract using two-binary multi-segment gradient elution liquid chromatography[J]. journal of separation science,2001,24(3):186-196. |
| APA | Xiao, HB,Liang, XM,&Lu, PC.(2001).Total analytical method for Radix astragali extract using two-binary multi-segment gradient elution liquid chromatography.journal of separation science,24(3),186-196. |
| MLA | Xiao, HB,et al."Total analytical method for Radix astragali extract using two-binary multi-segment gradient elution liquid chromatography".journal of separation science 24.3(2001):186-196. |
入库方式： OAI收割
来源：大连化学物理研究所
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