Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction
文献类型：期刊论文
| 作者 | Mei, SR; Xu, GW; Wu, CY |
| 刊名 | analytical letters
 |
| 出版日期 | 2001 |
| 卷号 | 34期号:12页码:2063-2076 |
| 关键词 | 8-hydroxydeoxyguanosine capillary electrophoresis solid-phase extraction urine sample |
| 英文摘要 | urinary 8-hydroxydeoxyguanosine (80hdg) has been considered as an excellent marker of individuals at high risk of developing cancer. until now, urinary 80hdg has largely been measured by high-performance liquid chromatography with electrochemical detection. a new method for the analysis of urinary 80hdg by high-performance capillary electrophoresis has been developed and optimized in our laboratory. a single step solid-phase extraction procedure was optimized and used for extracting 80hdg from human urine. separations were performed in an uncoated silica capillary (50 cm x 50 tm i.d.) using a p/ace mdq system with uv detection. the separation of 80hdg from interfering urinary matrix components is optimized with regard to ph, applied voltage, pressure injection time and concentration of sds in running buffer. the detection limit of this method is 0.4 mug/ml, the linear range is 0.8-500 mug/ml, the correlation coefficients levels is better than 0.999. the developed method is simple, fast and good reproducibility, furthermore, it requires a very small injection volumes and low costs of analysis, which makes it possible to provide a new noninvasive assay for an indirect measurement of oxidative dna damage. |
| WOS标题词 | science & technology ; physical sciences |
| 类目[WOS] | chemistry, analytical |
| 研究领域[WOS] | chemistry |
| 关键词[WOS] | oxidative dna-damage ; performance liquid-chromatography ; electrochemical detection ; 8-hydroxy-2'-deoxyguanosine ; assay ; biomarker ; 8-hydroxyguanine ; residue ; marker ; base |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000171530100006 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84649]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Wuhan Univ, Dept Chem, Wuhan 430072, Peoples R China 2.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog Res & Anal Ctr, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Mei, SR,Xu, GW,Wu, CY. Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction[J]. analytical letters,2001,34(12):2063-2076. |
| APA | Mei, SR,Xu, GW,&Wu, CY.(2001).Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction.analytical letters,34(12),2063-2076. |
| MLA | Mei, SR,et al."Analysis of urinary 8-hydroxydeoxyguanosine by capillary electrophoresis and solid-phase extraction".analytical letters 34.12(2001):2063-2076. |
入库方式： OAI收割
来源：大连化学物理研究所
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