Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach
文献类型：期刊论文
| 作者 | Xue, J; Wu, RG; Pan, YJ; Wang, SX; Qu, BW; Qin, Y; Shi, YQ; Zhang, CC; Li, R; Zhang, LY |
| 刊名 | ELECTROPHORESIS
 |
| 出版日期 | 2018 |
| 卷号 | 39期号:12页码:1466 |
| 关键词 | Forensic genetics GENOME MACHINE PGM Fusion primer FORENSIC APPLICATION Next-generation sequencing HUMAN IDENTIFICATION Short tandem repeat (STR) typing MISEQ POLYMORPHISM SYSTEM |
| ISSN号 | 0173-0835 |
| DOI | 10.1002/elps.201700429 |
| 文献子类 | Article |
| 英文摘要 | Massively parallel sequencing (MPS) technologies, also termed as next-generation sequencing (NGS), are becoming increasingly popular in study of short tandem repeats (STR). However, current library preparation methods are usually based on ligation or two-round PCR that requires more steps, making it time-consuming (about 2 days), laborious and expensive. In this study, a 16-plex STR typing system was designed with fusion primer strategy based on the Ion Torrent S5 XL platform which could effectively resolve the above challenges for forensic DNA database-type samples (bloodstains, saliva stains, etc.). The efficiency of this system was tested in 253 Han Chinese participants. The libraries were prepared without DNA isolation and adapter ligation, and the whole process only required approximately 5 h. The proportion of thoroughly genotyped samples in which all the 16 loci were successfully genotyped was 86% (220/256). Of the samples, 99.7% showed 100% concordance between NGS-based STR typing and capillary electrophoresis (CE)-based STR typing. The inconsistency might have been caused by off-ladder alleles and mutations in primer binding sites. Overall, this panel enabled the large-scale genotyping of the DNA samples with controlled quality and quantity because it is asimple, operation-friendly process flow that saves labor,time and costs. |
| WOS记录号 | WOS:000435778800006 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/26722]  |
| 专题 | 中国科学院过程工程研究所 |
| 推荐引用方式 GB/T 7714 | Xue, J,Wu, RG,Pan, YJ,et al. Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach[J]. ELECTROPHORESIS,2018,39(12):1466, 1473. |
| APA | Xue, J.,Wu, RG.,Pan, YJ.,Wang, SX.,Qu, BW.,...&Sun, Hongyu.(2018).Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach.ELECTROPHORESIS,39(12),1466. |
| MLA | Xue, J,et al."Integrated massively parallel sequencing of 15 autosomal STRs and Amelogenin using a simplified library preparation approach".ELECTROPHORESIS 39.12(2018):1466. |
入库方式： OAI收割
来源：过程工程研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。