Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp DY3
文献类型：期刊论文
| 作者 | Yuan, XH; Zhang, PF; Jiao, SM; Du, YG; Li, JJ; Yuan, Xianghua; Zhang, Pengfei; Jiao, Siming; Du, Yuguang; Li, Jian-Jun |
| 刊名 | INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
 |
| 出版日期 | 2018 |
| 卷号 | 116页码:100 |
| 关键词 | Cold-active cellulase COLD-ADAPTED ENDO-1,4-BETA-GLUCANASE pH and temperature-rate profile ERWINIA-CHRYSANTHEMI pH and thermal stability ANTARCTIC BACTERIUM ACTIVE CELLULASE EISENIA-FOETIDA GENE CLONING PURIFICATION BIOCONVERSION HALOPLANKTIS BIOREFINERY |
| ISSN号 | 0141-8130 |
| DOI | 10.1016/j.ijbiomac.2018.05.017 |
| 文献子类 | Article |
| 英文摘要 | Cold-active cellulases have received great attention for both industrial applications and fundamental research because of their high activity at low temperatures and their unique structural characters. In this study, the cold active endoglucanase CeIX from psychrotrophic Pseudoalteromonas sp. DY3 was successfully overexpressed in E. coli, partly purified and characterized in detail. CeIX showed the highest activity at pH 5.5, and exhibited moderate activity and superior pH stability over a wide pH range (pH 5.0-pH 9.0). It displayed the highest activity at 45 degrees C, and kept 34.7% residual activity even at 5 degrees C. It was stable below 35 degrees C and lost activity very quickly above 45 degrees C, which is consistent with its cold adaptability. The apparent kinetic parameters CeIX against CMC (carboxymethyl cellulose) were determined, with the K-m and k(cat) values of 6.4 mg/ml and 4.2 s(-1) respectively. Mn2+ and Co2+ enhanced the cellulolytic activity of CeIX by 28.8% and 20.6% respectively, whereas Pb2+ and Cu2+ inhibited its activity by 14.9% and 6.5% separately. The cold adaptation of CeIX is possibly due to the presence of the unusually long linker between the catalytic module and the cellulose-binding domain. (C) 2018 Published by Elsevier B.V. |
| WOS记录号 | WOS:000440120500013 |
| 源URL | [http://ir.ipe.ac.cn/handle/122111/26745]  |
| 专题 | 中国科学院过程工程研究所 |
| 推荐引用方式 GB/T 7714 | Yuan, XH,Zhang, PF,Jiao, SM,et al. Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp DY3[J]. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,2018,116:100, 105. |
| APA | Yuan, XH.,Zhang, PF.,Jiao, SM.,Du, YG.,Li, JJ.,...&Li, Jian-Jun.(2018).Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp DY3.INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES,116,100. |
| MLA | Yuan, XH,et al."Overexpression and biochemical characterization of a recombinant psychrophilic endocellulase from Pseudoalteromonas sp DY3".INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES 116(2018):100. |
入库方式： OAI收割
来源：过程工程研究所
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