Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides
文献类型：期刊论文
| 作者 | Zhao, Yiman1; Zhang, Lingyi1; Chu, Zhanying1; Xiong, Zhichao2; Zhang, Weibing1 |
| 刊名 | Analytical Methods
 |
| 出版日期 | 2017 |
| 卷号 | 9期号:3页码:443-449 |
| ISSN号 | 17599660 |
| DOI | 10.1039/c6ay02905a |
| 英文摘要 | For in-depth analysis of phosphorylated proteomics, the highly sensitive and selective capture of phosphopeptides from intricate biological samples is extremely significant. In this work, a late-model immobilized metal ion affinity chromatography (IMAC) material was fabricated and applied to enriching phosphopeptides from biological samples by abundant Ti4+ions, which were chelated to crosslinked chitosan (CS) loaded on the surface of magnetic graphene oxide (GO) (denoted as mag GO-CS-Ti4+). The high amount of crosslinked CS endowed the IMAC material with highly hydrophilic properties and abundant active sites for Ti4+ions on the surface. These characteristics ensure the great performance of mag GO-CS-Ti4+in the selective enrichment of phosphopeptides from the tryptic digest of β-casein with high selectivity (phosphopeptides to non-phosphopeptides at a molar ratio of 1 : 400), high sensitivity (0.5 fmol), large enrichment capacity (66.6 mg g-1), prominent phosphopeptide recovery (93.11%), and excellent reusability. What's more, mag GO-CS-Ti4+was applied to capturing phosphopeptides from real biological samples, human serum from both healthy people and patients and nonfat milk. The results successfully manifested that the mag GO-CS-Ti4+could be a great affinity material for the enrichment of low-abundant phosphopeptides from biological samples. © 2017 The Royal Society of Chemistry. |
| 源URL | [http://ir.sic.ac.cn/handle/331005/25935]  |
| 专题 | 中国科学院上海硅酸盐研究所 |
| 作者单位 | 1.Shanghai Key Laboratory of Functional Materials Chemistry, School of Chemistry and Molecular Engineering, East China University of Science and Technology, Shanghai; 200237, China; 2.State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences, Shanghai; 200050, China |
| 推荐引用方式 GB/T 7714 | Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,et al. Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides[J]. Analytical Methods,2017,9(3):443-449. |
| APA | Zhao, Yiman,Zhang, Lingyi,Chu, Zhanying,Xiong, Zhichao,&Zhang, Weibing.(2017).Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides.Analytical Methods,9(3),443-449. |
| MLA | Zhao, Yiman,et al."Ti4+-immobilized chitosan-coated magnetic graphene oxide for the highly selective enrichment of phosphopeptides".Analytical Methods 9.3(2017):443-449. |
入库方式： OAI收割
来源：上海硅酸盐研究所
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