Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase
文献类型：期刊论文
| 作者 | Wang, HL; Zou, HF; Ni, JY; Kong, L; Gao, S; Guo, BC |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2000-02-18 |
| 卷号 | 870期号:1-2页码:501-510 |
| 关键词 | Artemisia capillaris scoparone capillarisin |
| 英文摘要 | a method for the screening and analysis of biologically active compounds in traditional chinese medicine is proposed. affinity chromatography using a human serum albumin (hsa) stationary phase was applied to separate and analyze the bioactive compounds from artemisia capillaris thunb. five major peaks and several minor peaks were resolved based on their affinity to hsa, two of them were identified as scoparone (sco, 6,7-dimethoxycoumarin) and capillarisin (cap). cap shows a much higher affinity to hsa than sco. the effects of acetonitrile concentration, eluent ph, phosphate concentration and temperature on the retention behaviors of several major active components were also investigated, and it was found that hydrophobicity and eluent ph play major roles in changing retention values. the results demonstrate that the affinity chromatography with a hsa stationary phase is an effective way for analyzing and screening biologically active compounds in traditional chinese medicine. (c) 2000 elsevier science b.v. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; allosteric interaction ; protein-binding ; plasma-protein ; saiboku-to ; microdialysis ; drugs ; enantiomers ; warfarin |
| 收录类别 | ISTP ; SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000085455900054 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84985]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&D Ctr, Dalian 116011, Peoples R China 2.Cleveland State Univ, Dept Chem, Cleveland, OH 44115 USA |
| 推荐引用方式 GB/T 7714 | Wang, HL,Zou, HF,Ni, JY,et al. Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase[J]. journal of chromatography a,2000,870(1-2):501-510. |
| APA | Wang, HL,Zou, HF,Ni, JY,Kong, L,Gao, S,&Guo, BC.(2000).Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase.journal of chromatography a,870(1-2),501-510. |
| MLA | Wang, HL,et al."Fractionation and analysis of Artemisia capillaris Thunb. by affinity chromatography with human serum albumin as stationary phase".journal of chromatography a 870.1-2(2000):501-510. |
入库方式： OAI收割
来源：大连化学物理研究所
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