Separation of acidic compounds by strong anion-exchange capillary electrochromatography
文献类型：期刊论文
| 作者 | Ye, ML; Zou, HF; Liu, Z; Ni, JY |
| 刊名 | journal of chromatography a
 |
| 出版日期 | 2000-07-28 |
| 卷号 | 887期号:1-2页码:223-231 |
| 关键词 | electrochromatography ion-exchange electrochromatography mobile phase composition benzoic acids |
| 英文摘要 | separation of the acidic compounds in the ion-exchange capillary electrochromatograph (ie-cec) with strong anion-exchange packing as the stationary phase was studied. it was observed that the electroosmotic flow (eof) in strong anion-exchange cec moderately changed with increase of the eluent ionic strength and decrease of the eluent ph, but the acetonitrile concentration in the eluent had almost no effect on the eof. the eof in strong anion-exchange cec with eluent of low ph value was much larger than that in rp-cec with spherisorb-ods as the stationary phase. the retention of acidic compounds on the strong anion-exchange packing was relatively weak due to only partial ionization of them, and both chromatographic and electrophoretic processes contributed to separation. it was observed that the retention values of acidic compounds decreased with the increase of phosphate buffer and acetonitrile concentration in the eluent as well as the decrease of the applied voltage, and even the acidic compounds could elute before the void time. these factors also made an important contribution to the separation selectivity for tested acidic compounds, which could be separated rapidly with high column efficiency of more than 220 000 plates/m under the optimized separation conditions. (c) 2000 elsevier science bn. all rights reserved. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | performance liquid-chromatography ; zone electrophoresis ; gradient elution ; reversed-phase ; flow ; efficiency ; parameters |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000088315800015 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/84991]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Natl Chromatog R&A Ctr, Lab Chromatog, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ye, ML,Zou, HF,Liu, Z,et al. Separation of acidic compounds by strong anion-exchange capillary electrochromatography[J]. journal of chromatography a,2000,887(1-2):223-231. |
| APA | Ye, ML,Zou, HF,Liu, Z,&Ni, JY.(2000).Separation of acidic compounds by strong anion-exchange capillary electrochromatography.journal of chromatography a,887(1-2),223-231. |
| MLA | Ye, ML,et al."Separation of acidic compounds by strong anion-exchange capillary electrochromatography".journal of chromatography a 887.1-2(2000):223-231. |
入库方式： OAI收割
来源：大连化学物理研究所
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