Compared with free enzymes, immobilized enzymes are more robust and resistant to environmental
changes. In addition, with enhanced stability, immobilized enzymes can be separated from the reaction
mixture and used for repeated cycles. These advantages prompt their applications in various fields. This
review outlines the existing methods and easy separated support materials for enzymes immobilization.
After a brief introduction on the immobilized enzyme, the immobilization methods of adsorption,
entrapment, covalent attachment and cross-linking are discussed. The emphasis is given on the easy
separated support materials of magnetic nanoparticles (MNPs), membranes and capillary columns. An
outlook on the immobilized enzyme is given at last.