Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography
文献类型：期刊论文
| 作者 | Wang, HL; Kong, L; Zou, HF; Ni, JY; Zhang, YK |
| 刊名 | chromatographia
 |
| 出版日期 | 1999-10-01 |
| 卷号 | 50期号:7-8页码:439-445 |
| 关键词 | column liquid chromatography human serum albumin Angelica sinensis (Oliv.) Diels bioactive compounds |
| 英文摘要 | a novel strategy for the screening and analysis of biologically active compounds in traditional chinese medicine by molecular biochromatography is proposed. molecular biochromatography with human serum albumin (hsa) immobilized on silica as stationary phase was used to screen and analyse the bioactive compounds in the typical chinese medicine of angelica sinensis (oliv.) diels. ten peaks showed retention on this column, which is based on their affinity for hsa. ferulic acid and liguistilide were identified as the principal active components, which agrees very well with the results in the literature. a quality control method was also developed based on the simultaneous determination the concentrations of ferulic acid and liguistilide in solutions of angelica sinensis (oliv.) diels extracted with water and methanol. it was observed that the concentrations of ferulic acid and liguistilide in solution extracted with methanol were 2 and 53 times higher, respectively, than those with water. it was shown that molecular biochromatography is an effective way of analysing and screening biologically active compounds in traditional chinese medicine. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | human-serum-albumin ; performance liquid-chromatography ; allosteric interaction ; stationary phase ; protein-binding ; plasma-protein ; saiboku-to ; microdialysis ; enantiomers ; warfarin |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000084861000008 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/85585]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Natl Chromatog R & A Ctr, Dalian Inst Chem Phys, Dalian 116011, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, HL,Kong, L,Zou, HF,et al. Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography[J]. chromatographia,1999,50(7-8):439-445. |
| APA | Wang, HL,Kong, L,Zou, HF,Ni, JY,&Zhang, YK.(1999).Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography.chromatographia,50(7-8),439-445. |
| MLA | Wang, HL,et al."Screening and analysis of biologically active compounds in Angelica sinensis by molecular biochromatography".chromatographia 50.7-8(1999):439-445. |
入库方式： OAI收割
来源：大连化学物理研究所
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