Determination of total iron binding capacity of serum by capillary electrophoresis
文献类型：期刊论文
| 作者 | Ding, YS; Liu, LL; Ma, YF; Lin, BC |
| 刊名 | chromatographia
 |
| 出版日期 | 1999 |
| 卷号 | 49期号:1-2页码:71-74 |
| 关键词 | capillary electrophoresis serum analysis total iron binding capacity |
| 英文摘要 | a capillary electrophoresis (ce) technique for determining total iron binding capacity (tibc) of serum has been developed. the optimum serum pretreatment involves the following major steps: at first, saturate serum transferrin with fe+3; then, dissociate them completely after removing excess unbound fe. finally, complex the released iron with phenanthroline, a chromophore, to make suitable for the ce analysis. ammonium acetate (ph = 5.0) was used as ce background electrolyte solution. in this system, a good linear correlation coefficient was maintained over the range 0.5 similar to 10 mu m (r = 0.9979, n =12). seven adult serum samples were studied and the tibc parameters measured. in the present system, 10 similar to 30 mu l serum is sufficient for determination. the study shows that the ce technique described is a powerful method for rapid, efficient, sensitive and reliable analysis and hence particularly suitable for clinical application. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | transferrin receptor levels ; rheumatoid-arthritis ; performance ; separation ; proteins ; anemia |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000078308200012 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/86171]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Truman State Univ, Div Sci, Kirksville, MO 63501 USA |
| 推荐引用方式 GB/T 7714 | Ding, YS,Liu, LL,Ma, YF,et al. Determination of total iron binding capacity of serum by capillary electrophoresis[J]. chromatographia,1999,49(1-2):71-74. |
| APA | Ding, YS,Liu, LL,Ma, YF,&Lin, BC.(1999).Determination of total iron binding capacity of serum by capillary electrophoresis.chromatographia,49(1-2),71-74. |
| MLA | Ding, YS,et al."Determination of total iron binding capacity of serum by capillary electrophoresis".chromatographia 49.1-2(1999):71-74. |
入库方式： OAI收割
来源：大连化学物理研究所
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