Capillary electrophoresis study of human serum albumin binding to basic drugs
文献类型：期刊论文
| 作者 | Ding, YS; Zhu, XF; Lin, BC |
| 刊名 | chromatographia
 |
| 出版日期 | 1999-03-01 |
| 卷号 | 49期号:5-6页码:343-346 |
| 关键词 | capillary electrophoresis frontal analysis binding constants basic drugs human serum albumin |
| 英文摘要 | the applicability of capillary electrophoresis/frontal analysis (ce/fa) for determining the binding constants of the drugs propranolol (pro) and verapamil (ver) to human serum albumin (hsa) was investigated. after direct hydrodynamic injection of a drug-has mixture solution into a coated capillary (32 cm x 50 mu m i.d.), the basic drug was eluted as a zonal peak with a plateau region under condition of phosphate buffer (ph 7.4; ionic strength 0.17) at 12 kv positive running voltage. the unbound drug concentrations measured from the plateau peak heights had good correlation coefficients, r > 0.999. employing the scatchard plot, the klotz plot and nonlinear regression, the drug protein binding parameters, the binding constant and the number of binding sites on one protein molecule, were obtained. the binding constant obtained was compared to a reported equilibrium dialysis result and they are basically in good agreement. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; chemistry, analytical |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry |
| 关键词[WOS] | protein-binding ; zone electrophoresis ; frontal analysis ; constants |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000079216700019 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/86183]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China |
| 推荐引用方式 GB/T 7714 | Ding, YS,Zhu, XF,Lin, BC. Capillary electrophoresis study of human serum albumin binding to basic drugs[J]. chromatographia,1999,49(5-6):343-346. |
| APA | Ding, YS,Zhu, XF,&Lin, BC.(1999).Capillary electrophoresis study of human serum albumin binding to basic drugs.chromatographia,49(5-6),343-346. |
| MLA | Ding, YS,et al."Capillary electrophoresis study of human serum albumin binding to basic drugs".chromatographia 49.5-6(1999):343-346. |
入库方式： OAI收割
来源：大连化学物理研究所
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