Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis
文献类型：期刊论文
| 作者 | Zhang, B; Fung, YS; Lau, KM; Lin, BC |
| 刊名 | biomedical chromatography
 |
| 出版日期 | 1999-06-01 |
| 卷号 | 13期号:4页码:267-271 |
| 英文摘要 | capillary zone electrophoresis was used to monitor the interaction between bilirubin and human serum albumin. cord blood serum samples were injected directly into an uncoated fused-silica capillary (30 cm x 50 mu m i.d.) and separation was accomplished within 4 min without extensive sample pretreatment. the most suitable running buffer to separate free bilirubin from albumin bound bilirubin was found to contain 1.0 mmol/l edta, 5% acetonitrile and 15 mmol/l phosphate with ph adjusted to 8.4. approximately two bilirubin dianions could be bound per human serum albumin molecule in the cord blood serum. the binding constant was estimated to be 1.1 x 10(5) (l/mol) at 25 degrees c and ph 8.4. the peak area ratio of free bilirubin to total bilirubin can be used to determine the bilirubin binding capacity of cord blood serum for the concentration range of total bilirubin from 204 to 340 mu mol/l using 1:5 diluted cord blood seras. copyright (c) 1999 john wiley & sons, ltd. |
| WOS标题词 | science & technology ; life sciences & biomedicine ; physical sciences |
| 类目[WOS] | biochemical research methods ; biochemistry & molecular biology ; chemistry, analytical ; pharmacology & pharmacy |
| 研究领域[WOS] | biochemistry & molecular biology ; chemistry ; pharmacology & pharmacy |
| 关键词[WOS] | fluorescence |
| 收录类别 | SCI |
| 原文出处 | true |
| 语种 | 英语 |
| WOS记录号 | WOS:000081341200003 |
| 公开日期 | 2010-11-30 |
| 源URL | [http://159.226.238.44/handle/321008/86219]  |
| 专题 | 大连化学物理研究所_中国科学院大连化学物理研究所 |
| 作者单位 | 1.Chinese Acad Sci, Dalian Inst Chem Phys, Dalian 116023, Peoples R China 2.Univ Hong Kong, Dept Chem, Hong Kong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhang, B,Fung, YS,Lau, KM,et al. Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis[J]. biomedical chromatography,1999,13(4):267-271. |
| APA | Zhang, B,Fung, YS,Lau, KM,&Lin, BC.(1999).Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis.biomedical chromatography,13(4),267-271. |
| MLA | Zhang, B,et al."Bilirubin-human serum albumin interaction monitored by capillary zone electrophoresis".biomedical chromatography 13.4(1999):267-271. |
入库方式： OAI收割
来源：大连化学物理研究所
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。