Comparative Transcriptome Analysis Identifies Putative Genes Involved in the Biosynthesis of Xanthanolides in Xanthium strumarium L.
文献类型：期刊论文
| 作者 | Li, Yuanjun1,2; Gou, Junbo1,2; Chen, Fangfang1; Li, Changfu1; Zhang, Yansheng1 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-08-30 |
| 卷号 | 7页码:11 |
| 关键词 | Xanthium strumarium transcriptome sesquiterpene lactone biosynthesis CYP450 |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2016.01317 |
| 英文摘要 | Xanthiurn strurnariurn L. is a traditional Chinese herb belonging to the Asteraceae family. The major bioactive components of this plant are sesquiterpene lactones (STLs), which include the xanthanolides. To date, the biogenesis of xanthanolides, especially their downstream pathway, remains largely unknown. In X. strumarium, xanthanolides primarily accumulate in its glandular trichomes. To identify putative gene candidates involved in the biosynthesis of xanthanolides, three X. strumarium transcriptomes, which were derived from the young leaves of two different cultivars and the purified glandular trichomes from one of the cultivars, were constructed in this study. In total, 157 million clean reads were generated and assembled into 91,861 unigenes, of which 59,858 unigenes were successfully annotated. All the genes coding for known enzymes in the upstream pathway to the biosynthesis of xanthanolides were present in the X. strumarium transcriptomes. From a comparative analysis of the X. strurnarium transcriptomes, this study identified a number of gene candidates that are putatively involved in the downstream pathway to the synthesis of xanthanolides, such as four unigenes encoding CYP71 P450s, 50 unigenes for dehydrogenases, and 27 genes for acetyltransferases. The possible functions of these four CYP71 candidates are extensively discussed. In addition, 116 transcription factors that are highly expressed in X. strumarium glandular trichomes were also identified. Their possible regulatory roles in the biosynthesis of STLs are discussed. The global transcriptomic data for X. strumarium should provide a valuable resource for further research into the biosynthesis of xanthanolides. |
| 资助项目 | National Natural Science Foundation of China[31370339] ; Grant of One Hundred Talent Program of the CAS[29Y129441R0104444] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000382125500002 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/38]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuanjun,Gou, Junbo,Chen, Fangfang,et al. Comparative Transcriptome Analysis Identifies Putative Genes Involved in the Biosynthesis of Xanthanolides in Xanthium strumarium L.[J]. FRONTIERS IN PLANT SCIENCE,2016,7:11. |
| APA | Li, Yuanjun,Gou, Junbo,Chen, Fangfang,Li, Changfu,&Zhang, Yansheng.(2016).Comparative Transcriptome Analysis Identifies Putative Genes Involved in the Biosynthesis of Xanthanolides in Xanthium strumarium L..FRONTIERS IN PLANT SCIENCE,7,11. |
| MLA | Li, Yuanjun,et al."Comparative Transcriptome Analysis Identifies Putative Genes Involved in the Biosynthesis of Xanthanolides in Xanthium strumarium L.".FRONTIERS IN PLANT SCIENCE 7(2016):11. |
入库方式： OAI收割
来源：武汉植物园
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