Improvement of betulinic acid biosynthesis in yeast employing multiple strategies
文献类型：期刊论文
| 作者 | Zhou, Chen1,2; Li, Jing1,2; Li, Changfu1; Zhang, Yansheng1 |
| 刊名 | BMC BIOTECHNOLOGY
 |
| 出版日期 | 2016-08-17 |
| 卷号 | 16页码:9 |
| 关键词 | Betulinic acid Betulin Biosynthesis Saccharomyces cerevisiae |
| ISSN号 | 1472-6750 |
| DOI | 10.1186/s12896-016-0290-9 |
| 英文摘要 | Background: Betulinic acid (BA) is a lupane-type triterpene which has been considered as a promising agent to cure melanoma with no side effects. Considering that BA is naturally produced in small quantities in plants, we previously reported the success in engineering its production in yeast. In the present study, we attempted to improve BA biosynthesis in yeast by the use of different strategies. Results: We first isolated a gene encoding a lupeol C-28 oxidase (LO) from Betula platyphylla (designated as BPLO). BPLO showed a higher activity in BA biosynthesis compared to the previously reported LOs. In addition, two yeast platforms were compared for engineering the production of BA, which demonstrated that the WAT11 strain was better to host BA pathway than the CEN.PK strain. Based on the WAT11-chassiss, the Gal80p mutant was further constructed. The mutant produced 0.16 mg/L/OD600 of BA, which was 2.2 fold of that produced by the wild type strain (0.07 mg/L/OD600). Conclusions: This study reported our efforts to improve BA production in yeast employing multiple strategies, which included the identification of a novel LO enzyme with a higher activity in BA biosynthesis, the evaluation of two yeast strains for hosting the BA pathway, and the up-regulation of the expression of the BA pathway genes by managing yeast GAL gene regulon circuit. |
| 资助项目 | National Science and Technology Program of China during the 25th-Year Plan period[2012AA02A704] ; Hubei Renyue Pharmaceutical Co., LTD[492014-026053] |
| WOS研究方向 | Biotechnology & Applied Microbiology |
| 语种 | 英语 |
| WOS记录号 | WOS:000381730800001 |
| 出版者 | BIOMED CENTRAL LTD |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/39]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhou, Chen,Li, Jing,Li, Changfu,et al. Improvement of betulinic acid biosynthesis in yeast employing multiple strategies[J]. BMC BIOTECHNOLOGY,2016,16:9. |
| APA | Zhou, Chen,Li, Jing,Li, Changfu,&Zhang, Yansheng.(2016).Improvement of betulinic acid biosynthesis in yeast employing multiple strategies.BMC BIOTECHNOLOGY,16,9. |
| MLA | Zhou, Chen,et al."Improvement of betulinic acid biosynthesis in yeast employing multiple strategies".BMC BIOTECHNOLOGY 16(2016):9. |
入库方式： OAI收割
来源：武汉植物园
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