Differential Molecular Responses of Rapeseed Cotyledons to Light and Dark Reveal Metabolic Adaptations toward Autotrophy Establishment
文献类型：期刊论文
| 作者 | Xi, Chen5; Yi, Bin4; Yang, Pingfang1,2; Fu, Jinlei1,3; Damaris, Rebecca N.1,3; He, Dongli1; Tu, Jinxing4; Fu, Tingdong4 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-07-14 |
| 卷号 | 7页码:15 |
| 关键词 | cotyledons light proteome metabolome LC-MS/MS GC-MS Brassica napus |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2016.00988 |
| 英文摘要 | Photosynthesis competent autotrophy is established during the postgerminative stage of plant growth. Among the multiple factors, light plays a decisive role in the switch from heterotrophic to autotrophic growth. Under dark conditions, the rapeseed hypocotyl extends quickly with an apical hook, and the cotyledon is yellow and folded, and maintains high levels of the isocitrate lyase (ICL). By contrast, in the light, the hypocotyl extends slowly, the cotyledon unfolds and turns green, the ICL content changes in parallel with cotyledon greening. To reveal metabolic adaptations during the establishment of postgerminative autotrophy in rapeseed, we conducted comparative proteomic and metabolomic analyses of the cotyledons of seedlings grown under light versus dark conditions. Under both conditions, the increase in proteases, fatty acid 8-oxidation and glyoxylate-cycle related proteins was accompanied by rapid degradation of the stored proteins and lipids with an accumulation of the amino acids. While light condition partially retarded these conversions. Light significantly induced the expression of chlorophyll-binding and photorespiration related proteins, resulting in an increase in reducing-sugars. However, the levels of some chlorophyllide conversion. Calvin-cycle and photorespiration related proteins also accumulated in dark grown cotyledons, implying that the transition from heterotrophy to autotrophy is programmed in the seed rather than induced by light. Various anti-stress systems, e.g., redox related proteins, salicylic acid, proline and chaperones, were employed to decrease oxidative stress, which was mainly derived from lipid oxidation or photorespiration, under both conditions. This study provides a comprehensive understanding of the differential molecular responses of rapeseed cotyledons to light and dark conditions, which will facilitate further study on the complex mechanism underlying the transition from heterotrophy to autotrophy. |
| 资助项目 | National Key Laboratory of Crop Genetic Improvement, China[ZK201012] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000379568300001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/47]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yi, Bin; Yang, Pingfang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Peoples R China 2.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan, Peoples R China 3.Univ Chinese Acad Sci, Beijing, Peoples R China 4.Huazhong Agr Univ, Natl Ctr Rapeseed Improvement Wuhan, Natl Key Lab Crop Genet Improvement, Wuhan, Peoples R China 5.Wuhan Inst Biotechnol, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Xi, Chen,Yi, Bin,Yang, Pingfang,et al. Differential Molecular Responses of Rapeseed Cotyledons to Light and Dark Reveal Metabolic Adaptations toward Autotrophy Establishment[J]. FRONTIERS IN PLANT SCIENCE,2016,7:15. |
| APA | Xi, Chen.,Yi, Bin.,Yang, Pingfang.,Fu, Jinlei.,Damaris, Rebecca N..,...&Fu, Tingdong.(2016).Differential Molecular Responses of Rapeseed Cotyledons to Light and Dark Reveal Metabolic Adaptations toward Autotrophy Establishment.FRONTIERS IN PLANT SCIENCE,7,15. |
| MLA | Xi, Chen,et al."Differential Molecular Responses of Rapeseed Cotyledons to Light and Dark Reveal Metabolic Adaptations toward Autotrophy Establishment".FRONTIERS IN PLANT SCIENCE 7(2016):15. |
入库方式： OAI收割
来源：武汉植物园
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