An Alternative Pathway for Formononetin Biosynthesis in Pueraria lobata
文献类型：期刊论文
| 作者 | Li, Jia1; Li, Changfu1; Gou, Junbo1,2; Wang, Xin1; Fan, Rongyan1,2; Zhang, Yansheng1 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-06-14 |
| 卷号 | 7页码:12 |
| 关键词 | hairy root isoflavone 4'-O-methylation O-methyltransferase Pueraria lobate |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2016.00867 |
| 英文摘要 | The O-methylation is an important tailing process in Pueraria lobata isoflavone metabolism, but the molecular mechanism governing it remains not elucidated. This manuscript describes the mining of key O-methyltransferases (OMTs) involved in the process. Using our previously constructed P lobata transcriptome, the OMT candidates were searched, extensively analyzed, and their functions were investigated by expression in yeast, Escherichla coil, or GIycine max hairy roots. Here, we report the identification of the key OMT gene responsible for formononetin production in P lobata (designated as PIOMT9). PIOMT9 primarily functions as an isoflavone-specific 4'-O-methyltransferase, although it shows high sequence identities with isoflavone 7-O-methyltransferases. Moreover, unlike the previously reported OMTs that catalyze the 4'-O-methylation for formononetin biosynthesis at the isoflavanone stage, PIOMT9 performs this modifying step at the isoflavone level, using daidzein rather than 2,7,4'-trihydroxy-isoflavanone as the substrate. Gene expression analyses and metabolite profiling supported its proposed roles in P. lobata. Using the system of transgenic G. max hairy roots, the role of PIOMT9 in the biosynthesis of formononetin was further demonstrated in vivo. |
| 资助项目 | National Natural Science Foundation of China[31300258] ; National Natural Science Foundation of China[31170284] ; One Hundred Talent Program of Chinese Academy of Science to Yansheng Zhang[Y129441R01] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000377660000001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/59]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Hubei, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jia,Li, Changfu,Gou, Junbo,et al. An Alternative Pathway for Formononetin Biosynthesis in Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2016,7:12. |
| APA | Li, Jia,Li, Changfu,Gou, Junbo,Wang, Xin,Fan, Rongyan,&Zhang, Yansheng.(2016).An Alternative Pathway for Formononetin Biosynthesis in Pueraria lobata.FRONTIERS IN PLANT SCIENCE,7,12. |
| MLA | Li, Jia,et al."An Alternative Pathway for Formononetin Biosynthesis in Pueraria lobata".FRONTIERS IN PLANT SCIENCE 7(2016):12. |
入库方式： OAI收割
来源：武汉植物园
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