Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata
文献类型：期刊论文
| 作者 | Li, Jia1; Li, Changfu1; Gou, Junbo1,2; Zhang, Yansheng1 |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-06-06 |
| 卷号 | 7页码:8 |
| 关键词 | methyltransferase methyl jasmonate isoflavone 3 '-O-methylation Pueraria lobata |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2016.00793 |
| 英文摘要 | Pueraria lobate roots accumulate 3'-, 4'- and 7-O-methylated isoflavones and many of these methylated compounds exhibit various pharmacological activities. Either the 4'- or 7-O-methylation activity has been investigated at molecular levels in several legume species. However, the gene encoding the isoflavone 3'-O-methyltransferase (OMT) has not yet been isolated from any plant species. In this study, we reported the first cDNA encoding the isoflavone 3'-OMT from P. lobata (designated PIOMT4). Heterologous expressions in yeast and Escherichia coli cells showed that the gene product exhibits an enzyme activity to methylate the 3'-hydroxy group of the isoflavone substrate. The transcript abundance of PIOMT4 matches well with its enzymatic product in different organs of P lobata and in the plant roots in response to methyl jasmonate elicitation. Integration of the biochemical with metabolic and transcript data supported the proposed function of PIOMT4. The identification of PIOMT4 would not only help to understand the isoflavonoid metabolism in P lobata but also potentially provide an enzyme catalyst for methylating existing drug candidates to improve their hydrophobicity. |
| 资助项目 | National Natural Science Foundation of China[31300258] ; National Natural Science Foundation of China[31170284] ; One Hundred Talent Program of the CAS[29Y129441R0104444] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000377071100001 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/63]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Jia,Li, Changfu,Gou, Junbo,et al. Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2016,7:8. |
| APA | Li, Jia,Li, Changfu,Gou, Junbo,&Zhang, Yansheng.(2016).Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata.FRONTIERS IN PLANT SCIENCE,7,8. |
| MLA | Li, Jia,et al."Molecular Cloning and Functional Characterization of a Novel Isoflavone 3 '-O-methyltransferase from Pueraria lobata".FRONTIERS IN PLANT SCIENCE 7(2016):8. |
入库方式： OAI收割
来源：武汉植物园
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