Molecular Cloning and Functional Characterization of a Novel (Iso)flavone 4 ',7-O-diglucoside Glucosyltransferase from Pueraria lobata
文献类型：期刊论文
| 作者 | Wang, Xin; Fan, Rongyan; Zhang, Yansheng; Li, Changfu; Li, Jia |
| 刊名 | FRONTIERS IN PLANT SCIENCE
 |
| 出版日期 | 2016-03-31 |
| 卷号 | 7页码:11 |
| 关键词 | glucosyltransferase isoflavone flavone 4 ',7-O-di-glucosylation 4 '-O-glucosylation Pueraria lobate |
| ISSN号 | 1664-462X |
| DOI | 10.3389/fpls.2015.00387 |
| 英文摘要 | Pueraria lobate roots accumulate a rich source of isoflavonoid glycosides, including 7-O- and 4'-O-mono-glucosides, and 4',7-O-diglucosides, which have numerous human health benefits. Although, isoflavonoid 7-O-glucosyltranferases (7-O-UGTs) have been well -characterized at molecular levels in legume plants, genes, or enzymes that are required for isoflavonoid 4'-O- and 4',7-O-glucosylation have not been identified in P lobata to date. Especially for the 4',7-O-di-glucosylations, the genetic control for this tailing process has never been elucidated from any plant species. Through transcriptome mining, we describe here the identification and characterization of a novel UGT (designated PIUGT2) governing the isoflavonoid 4',7-O-di-glucosylations in P lobata. Biochemical roles of PIUGT2 were assessed by in vitro assays with PIUGT2 protein produced in Escherichia coli and analyzed for its qualitative substrate specificity. PIUGT2 was active with various (iso)flavonoid acceptors, catalyzing consecutive glucosylation activities at their O-4' and O-7 positions. PIUGT2 was most active with genistein, a general isoflavone in legume plants. Real-time PCR analysis showed that PIUGT2 is preferentially transcribed in roots relative to other organs of P lobata, which is coincident with the accumulation pattern of 4'-O-glucosides and 4',7-Odiglucosides in P lobata. The identification of PIUGT2 would help to decipher the P lobata isoflavonoid glucosylations in vivo and may provide a useful enzyme catalyst for an efficient biotransformation of isoflavones or other natural products for food or pharmacological purposes. |
| 资助项目 | National Natural Science Foundation of China[31170284] ; One Hundred Talent Program of the CAS[29Y129441R0104444] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000373261200002 |
| 出版者 | FRONTIERS MEDIA SA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/99]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | Chinese Acad Sci, Wuhan Bot Garden, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan, Peoples R China |
| 推荐引用方式 GB/T 7714 | Wang, Xin,Fan, Rongyan,Zhang, Yansheng,et al. Molecular Cloning and Functional Characterization of a Novel (Iso)flavone 4 ',7-O-diglucoside Glucosyltransferase from Pueraria lobata[J]. FRONTIERS IN PLANT SCIENCE,2016,7:11. |
| APA | Wang, Xin,Fan, Rongyan,Zhang, Yansheng,Li, Changfu,&Li, Jia.(2016).Molecular Cloning and Functional Characterization of a Novel (Iso)flavone 4 ',7-O-diglucoside Glucosyltransferase from Pueraria lobata.FRONTIERS IN PLANT SCIENCE,7,11. |
| MLA | Wang, Xin,et al."Molecular Cloning and Functional Characterization of a Novel (Iso)flavone 4 ',7-O-diglucoside Glucosyltransferase from Pueraria lobata".FRONTIERS IN PLANT SCIENCE 7(2016):11. |
入库方式： OAI收割
来源：武汉植物园
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