Identification and Functional Characterization of Sesquiterpene Synthases from Xanthium strumarium
文献类型：期刊论文
| 作者 | Li, Yuanjun1,2; Chen, Fangfang1; Li, Zhenqiu3; Li, Changfu1; Zhang, Yansheng1 |
| 刊名 | PLANT AND CELL PHYSIOLOGY
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 57期号:3页码:630-641 |
| 关键词 | Sesquiterpene lactone Sesquiterpene synthase Xanthium strumarium |
| ISSN号 | 0032-0781 |
| DOI | 10.1093/pcp/pcw019 |
| 英文摘要 | Xanthium strumarium synthesizes various pharmacologically active sesquiterpenes. The molecular characterization of sesquiterpene biosynthesis inX. strumarium has not been reported so far. In this study, the cDNAs coding for three sesquiterpene synthases (designated as XsTPS1, XsTPS2 and XsTPS3) were isolated using theX. strumarium transcriptome that we recently constructed. XsTPS1, XsTPS2 and XsTPS3 were revealed to have primary activities forming germacrene D, guaia-4,6-diene and germacrene A, respectively, by either ectopic expression in yeast cells or purified recombinant protein-based in vitro assays. Quantitative real-time PCRs and metabolite analysis for the different plant parts showed that the transcript abundance ofXsTPS1-XsTPS3 is consistent with the accumulation pattern of their enzymatic products, supporting their biochemical functions in vivo. In particular, we discovered that none of the XsTPS2 product, guaia-4,6-diene, can be detected in one of theX. strumarium cultivars used in this study (it was named the Hubei-cultivar), in which a natural deletion of two A bases in the XsTPS2 cDNA disrupts its activity, which further confirmed the proposed biochemical role of XsTPS2 inX. strumarium in vivo. |
| 资助项目 | National Natural Science Foundation of China[31270341] ; National Natural Science Foundation of China[31370339] |
| WOS研究方向 | Plant Sciences ; Cell Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000374572400016 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/102]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Zhang, Yansheng |
| 作者单位 | 1.Chinese Acad Sci, CAS Key Lab Plant Germplasm Enhancement & Special, Wuhan Bot Garden, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Hebei Univ, Coll Life Sci, Baoding 071002, Peoples R China |
| 推荐引用方式 GB/T 7714 | Li, Yuanjun,Chen, Fangfang,Li, Zhenqiu,et al. Identification and Functional Characterization of Sesquiterpene Synthases from Xanthium strumarium[J]. PLANT AND CELL PHYSIOLOGY,2016,57(3):630-641. |
| APA | Li, Yuanjun,Chen, Fangfang,Li, Zhenqiu,Li, Changfu,&Zhang, Yansheng.(2016).Identification and Functional Characterization of Sesquiterpene Synthases from Xanthium strumarium.PLANT AND CELL PHYSIOLOGY,57(3),630-641. |
| MLA | Li, Yuanjun,et al."Identification and Functional Characterization of Sesquiterpene Synthases from Xanthium strumarium".PLANT AND CELL PHYSIOLOGY 57.3(2016):630-641. |
入库方式： OAI收割
来源：武汉植物园
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