RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway
文献类型：期刊论文
| 作者 | Chan, Zhulong1,2,3; Wang, Yanping1; Cao, Minjie2,3; Gong, Yuehua4,5; Mu, Zixin4,6; Wang, Haiqing4,7; Hu, Yuanlei4,8; Deng, Xin4,9; He, Xin-Jian10; Zhu, Jian-Kang2,3,4 |
| 刊名 | NEW PHYTOLOGIST
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 209期号:4页码:1527-1539 |
| 关键词 | C-REPEAT-BINDING FACTOR (CBF) cold Pol II RNA-directed DNA methylation RNA-DIRECTED DNA METHYLATION 4 (RDM4) transcriptome |
| ISSN号 | 0028-646X |
| DOI | 10.1111/nph.13727 |
| 英文摘要 | The C-REPEAT-BINDING FACTOR (CBF) pathway has important roles in plant responses to cold stress. How the CBF genes themselves are activated after cold acclimation remains poorly understood. In this study, we characterized cold tolerance of null mutant of RNA-DIRECTED DNA METHYLATION 4 (RDM4), which encodes a protein that associates with RNA polymerases Pol V and Pol II, and is required for RNA-directed DNA methylation (RdDM) in Arabidopsis. The results showed that dysfunction of RDM4 reduced cold tolerance, as evidenced by decreased survival and increased electrolyte leakage. Mutation of RDM4 resulted in extensive transcriptomic reprogramming. CBFs and CBF regulon genes were down-regulated in rdm4 but not nrpe1 (the largest subunit of PolV) mutants, suggesting that the role of RDM4 in cold stress responses is independent of the RdDM pathway. Overexpression of RDM4 constitutively increased the expression of CBFs and regulon genes and decreased cold-induced membrane injury. A great proportion of genes affected by rdm4 overlapped with those affected by CBFs. Chromatin immunoprecipitation results suggested that RDM4 is important for Pol II occupancy at the promoters of CBF2 and CBF3. We present evidence of a considerable role for RDM4 in regulating gene expression at low temperature, including the CBF pathway in Arabidopsis. |
| 资助项目 | National Natural Science Foundation of China[31370302] ; Knowledge Innovative Key Program of the Chinese Academy of Sciences[54Y154761O01076] ; Knowledge Innovative Key Program of the Chinese Academy of Sciences[Y329631O0263] ; Chinese Academy of Sciences ; US National Institutes of Health[R01GM059138] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000373379800022 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/105]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Chan, Zhulong; Zhu, Jian-Kang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei Province, Peoples R China 2.Chinese Acad Sci, Shanghai Ctr Plant Stress Biol, Shanghai 200032, Peoples R China 3.Chinese Acad Sci, Inst Plant Physiol & Ecol, Shanghai 200032, Peoples R China 4.Purdue Univ, Dept Hort & Landscape Architecture, W Lafayette, IN 47906 USA 5.Yibin Univ, Coll Life Sci & Food Engn, Yibin 644000, Sichuan, Peoples R China 6.Northwest A&F Univ, Coll Life Sci, Yangling 712100, Shaanxi, Peoples R China 7.Chinese Acad Sci, Northwest Plateau Inst Biol, Key Lab Adaptat & Evolut Plateau Biota, Xining 810001, Qinghai, Peoples R China 8.Peking Univ, Coll Life Sci, Beijing 100871, Peoples R China 9.Chinese Acad Sci, Inst Bot, Key Lab Plant Resources, Beijing 100093, Peoples R China 10.Natl Inst Biol Sci, Beijing 102206, Peoples R China |
| 推荐引用方式 GB/T 7714 | Chan, Zhulong,Wang, Yanping,Cao, Minjie,et al. RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway[J]. NEW PHYTOLOGIST,2016,209(4):1527-1539. |
| APA | Chan, Zhulong.,Wang, Yanping.,Cao, Minjie.,Gong, Yuehua.,Mu, Zixin.,...&Zhu, Jian-Kang.(2016).RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway.NEW PHYTOLOGIST,209(4),1527-1539. |
| MLA | Chan, Zhulong,et al."RDM4 modulates cold stress resistance in Arabidopsis partially through the CBF-mediated pathway".NEW PHYTOLOGIST 209.4(2016):1527-1539. |
入库方式： OAI收割
来源：武汉植物园
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