Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH
文献类型：期刊论文
| 作者 | Zhang, Hui1,2; He, Dongli1; Yu, Jianlan3; Li, Ming1; Damaris, Rebecca Njeri1; Gupta, Ravi4; Kim, Sun Tae4; Yang, Pingfang1 |
| 刊名 | PROTEOMICS
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 16期号:6页码:989-1000 |
| 关键词 | Biotin hydrazide Germination Peroxiredoxin Plant proteomics Protein carbonylation SWATH |
| ISSN号 | 1615-9853 |
| DOI | 10.1002/pmic.201500248 |
| 英文摘要 | Seed germination is an important aspect of the plant life cycle, during which, reactive oxygen species (ROS) accumulate. The accumulation of ROS results in an increase in protein oxidation of which carbonylation is the most canonical one. However, there is insufficient information concerning protein oxidation, especially carbonylation and its contribution to seed germination. In this study, biotin hydrazide labeled chromatography combined with sequential window acquisition of all theoretical fragment ion spectra (SWATH) method was used to analyze the dynamic pattern of protein carbonylation in rice embryos during germination. A total of 1872 unique proteins were quantified, among which 288 carbonylated peptides corresponding to 144 proteins were determined based on the filtering through mass shifts of modified amino acids. In addition, 66 carbonylated proteins were further analyzed based on their carbonylation intensity in four stages of germination. These identified carbonylated proteins were mainly involved in maintaining the levels of ROS, abscisic acid and seed reserves. Remarkably, a peroxiredoxin was found with 23 unique carbonylated peptides, and the expression of which was consistent with its increased activity. This study describes the dynamic pattern of carbonylated proteins during seed germination, and may help to further understand the biochemical mechanisms on this process. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31271805] ; Sino-Africa Joint Research Project[SAJC201324] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000372976400009 |
| 出版者 | WILEY-BLACKWELL |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/107]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Pingfang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.AB Sciex, Asia Pacific Applicat Support Ctr, Shanghai, Peoples R China 4.Pusan Natl Univ, Coll Nat Resources & Life Sci, Dept Plant Biosci, Miryang, South Korea |
| 推荐引用方式 GB/T 7714 | Zhang, Hui,He, Dongli,Yu, Jianlan,et al. Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH[J]. PROTEOMICS,2016,16(6):989-1000. |
| APA | Zhang, Hui.,He, Dongli.,Yu, Jianlan.,Li, Ming.,Damaris, Rebecca Njeri.,...&Yang, Pingfang.(2016).Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH.PROTEOMICS,16(6),989-1000. |
| MLA | Zhang, Hui,et al."Analysis of dynamic protein carbonylation in rice embryo during germination through AP-SWATH".PROTEOMICS 16.6(2016):989-1000. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。