热门
Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed
文献类型：期刊论文
| 作者 | He, Dongli1; Wang, Qiong1,2; Li, Ming1; Damaris, Rebecca Njeri1,2; Yi, Xingling3; Cheng, Zhongyi3; Yang, Pingfang1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2016-03-01 |
| 卷号 | 15期号:3页码:879-890 |
| 关键词 | post-translational modification acetyllysine succinyllysine metabolism seed germination rice |
| ISSN号 | 1535-3893 |
| DOI | 10.1021/acs.jproteome.5b00805 |
| 英文摘要 | Regulation of rice seed germination has been shown to mainly occur at post-transcriptional levels, of which the changes on proteome status is a major one. Lysine acetylation and succinylation are two prevalent protein post-translational modifications (PTMs) involved in multiple biological processes, especially for metabolism regulation. To investigate the potential mechanism controlling metabolism regulation in rice seed germination, we performed the lysine acetylation and succinylation analyses simultaneously. Using high-accuracy nano-LC MS/MS in combination with the enrichment of lysine acetylated or succinylated peptides from digested embryonic proteins of 24 h after imbibition (HAI) rice seed, a total of 699 acetylated sites from 389 proteins and 665 succinylated sites from 261 proteins were identified. Among these modified lysine sites, 133 sites on 78 proteins were commonly modified by two PTMs. The overlapped PTM sites were more likely to be in polar acidic/basic amino acid regions and exposed on the protein surface. Both of the acetylated and succinylated proteins cover nearly all aspects of cellular functions. Ribosome complex and glycolysis/ gluconeogenesis-related proteins were significantly enriched in both acetylated and succinylated protein profiles through KEGG enrichment and protein protein interaction network analyses. The acetyl-CoA and succinyl-CoA metabolism-related enzymes were found to be extensively modified by both modifications, implying the functional interaction between the two PTMs. This study provides a rich resource to examine the modulation of the two PTMs on the metabolism pathway and other biological processes in germinating rice seed. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31101213] ; National Natural Science Foundation of China (NSFC)[31271805] ; 100 talents program of Chinese Academy of Sciences ; Sino-Africa Joint Research Project[SAJC201324] |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000371754100021 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/109]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Pingfang |
| 作者单位 | 1.Chinese Acad Sci, Sinoafrican Joint Res Ctr, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Jingjie PTM Biolabs Hangzhou Co Ltd, Hangzhou 310018, Zhejiang, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Dongli,Wang, Qiong,Li, Ming,et al. Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed[J]. JOURNAL OF PROTEOME RESEARCH,2016,15(3):879-890. |
| APA | He, Dongli.,Wang, Qiong.,Li, Ming.,Damaris, Rebecca Njeri.,Yi, Xingling.,...&Yang, Pingfang.(2016).Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed.JOURNAL OF PROTEOME RESEARCH,15(3),879-890. |
| MLA | He, Dongli,et al."Global Proteome Analyses of Lysine Acetylation and Succinylation Reveal the Widespread Involvement of both Modification in Metabolism in the Embryo of Germinating Rice Seed".JOURNAL OF PROTEOME RESEARCH 15.3(2016):879-890. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。