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Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis

文献类型：期刊论文

作者Yuan, Yangyang1,4; Xin, Haiping1,2,3; Li, Shaohua1,2,3; Gao, Yingying1,4; Zhang, Langlang1,4; Karungo, Sospeter Karanja1,4; Fang, Linchuan1,4
刊名PLANT CELL REPORTS
出版日期2016-03-01
卷号35期号:3页码:655-666
关键词GRAS transcription factor VaPAT1 Vitis amurensis Abiotic stress Gibberellic acid (GA) Stress-related genes
ISSN号0721-7714
DOI10.1007/s00299-015-1910-x
英文摘要The plant-specific GRAS transcription factor family regulates diverse processes involved in plant growth, development and stress responses. In this study, VaPAT1, a GRAS gene from Vitis amurensis was isolated and functionally characterized. Sequence alignment and phylogenetic analysis showed that VaPAT1 has a high sequence identity to CmsGRAS and OsCIGR1, which belong to PAT1 branch of GRAS family and function in stress resistance. The transcription of VaPAT1 was markedly induced by stress-related phytohormone abscisic acid (ABA) and various abiotic stress treatments such as cold, drought and high salinity, however, it was repressed by exogenous gibberellic acid (GA) application. Overexpression of VaPAT1 increased the cold, drought and high salinity tolerance in transgenic Arabidopsis. When compared with wild type (WT) seedlings, the VaPAT1-overexpression lines accumulated higher levels of proline and soluble sugar under these stress treatments. Moreover, stress-related genes such as AtSIZ1, AtCBF1, AtATR1/MYB34, AtMYC2, AtCOR15A, AtRD29A and AtRD29B showed higher expression levels in VaPAT1 transgenic lines than in WT Arabidopsis under normal growth conditions. Together, our results indicated that VaPAT1 functions as a positive transcriptional regulator involved in grapevine abiotic stress responses.
资助项目National Science Foundation of China (NSFC)[31130047] ; National Science Foundation of China (NSFC)[31471857] ; National Key Technology R&D Program of the Ministry of Science and Technology during the Twelfth Five-year Plan Period[2013BAD02B04-1] ; Youth Innovation Promotion Association of CAS[2015281]
WOS研究方向Plant Sciences
语种英语
WOS记录号WOS:000371241100013
出版者SPRINGER
源URL[http://202.127.146.157/handle/2RYDP1HH/112]  
专题中国科学院武汉植物园
通讯作者Xin, Haiping
作者单位1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China
2.Chinese Acad Sci, Beijing Key Lab Grape Sci & Enol, Inst Bot, Beijing 100093, Peoples R China
3.Chinese Acad Sci, CAS Key Lab Plant Resources, Inst Bot, Beijing 100093, Peoples R China
4.Univ Chinese Acad Sci, 19A Yuquanlu, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Yuan, Yangyang,Xin, Haiping,Li, Shaohua,et al. Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis[J]. PLANT CELL REPORTS,2016,35(3):655-666.
APA Yuan, Yangyang.,Xin, Haiping.,Li, Shaohua.,Gao, Yingying.,Zhang, Langlang.,...&Fang, Linchuan.(2016).Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis.PLANT CELL REPORTS,35(3),655-666.
MLA Yuan, Yangyang,et al."Overexpression of VaPAT1, a GRAS transcription factor from Vitis amurensis, confers abiotic stress tolerance in Arabidopsis".PLANT CELL REPORTS 35.3(2016):655-666.

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来源：武汉植物园

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