Development of expressed sequence tag-based microsatellite markers for the critically endangered Isoetes sinensis (Isoetaceae) based on transcriptome analysis
文献类型：期刊论文
| 作者 | Gichira, A. W.1,2; Long, Z. C.1,2; Wang, Q. F.1; Chen, J. M.1; Liao, K.1 |
| 刊名 | GENETICS AND MOLECULAR RESEARCH
 |
| 出版日期 | 2016 |
| 卷号 | 15期号:3页码:10 |
| 关键词 | De novo assembly EST-SSR Isoetes sinensis Microsatellite RNA-sequencing Transcriptome |
| ISSN号 | 1676-5680 |
| DOI | 10.4238/gmr.15038497 |
| 英文摘要 | Isoetes sinensis is a critically endangered quillwort. To facilitate studies on the conservation genetics of this species, we developed expressed sequence tag-simple sequence repeat (EST-SSR) markers. A total of 50,063 unigenes were predicted by transcriptome sequencing, 5294 (10.6%) of which significantly matched 3011 Gene Ontology annotations and 2363 were assigned to Kyoto Encyclopedia of Genes and Genomes metabolic pathways. Most of these (2297) were involved in metabolism. A total of 1982 SSR motifs were identified, with trinucleotides being the dominant repeat motif, and 1438 (72.6%) SSR primers were designed. Eighteen randomly selected primer pairs were used to genotype 24 I. sinensis accessions, which confirmed the suitability of these novel markers for molecular studies of I. sinensis. The heterozygosity index value ranged between 0.0799 and 0.9106, while the Shannon-Wiener diversity index value ranged between 0.1732 and 2.5589. The EST-SSRs reported in this study are linked to genic sequences, and are therefore ideal for investigating the evolutionary history of I. sinensis. These markers, together with the large EST dataset generated in this study, will greatly facilitate conservation genetic studies of I. sinensis. |
| 资助项目 | National Natural Science Foundation of China[31200170] ; National Natural Science Foundation of China[31570220] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Genetics & Heredity |
| 语种 | 英语 |
| WOS记录号 | WOS:000384881300071 |
| 出版者 | FUNPEC-EDITORA |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/143]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Liao, K. |
| 作者单位 | 1.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China |
| 推荐引用方式 GB/T 7714 | Gichira, A. W.,Long, Z. C.,Wang, Q. F.,et al. Development of expressed sequence tag-based microsatellite markers for the critically endangered Isoetes sinensis (Isoetaceae) based on transcriptome analysis[J]. GENETICS AND MOLECULAR RESEARCH,2016,15(3):10. |
| APA | Gichira, A. W.,Long, Z. C.,Wang, Q. F.,Chen, J. M.,&Liao, K..(2016).Development of expressed sequence tag-based microsatellite markers for the critically endangered Isoetes sinensis (Isoetaceae) based on transcriptome analysis.GENETICS AND MOLECULAR RESEARCH,15(3),10. |
| MLA | Gichira, A. W.,et al."Development of expressed sequence tag-based microsatellite markers for the critically endangered Isoetes sinensis (Isoetaceae) based on transcriptome analysis".GENETICS AND MOLECULAR RESEARCH 15.3(2016):10. |
入库方式： OAI收割
来源：武汉植物园
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