Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination
文献类型：期刊论文
| 作者 | He, Dongli1; Wang, Qiong1,2; Wang, Kun3; Yang, Pingfang1 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015-12-17 |
| 卷号 | 10期号:12页码:21 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0145424 |
| 英文摘要 | The first 24 hours after imbibition (HAI) is pivotal for rice seed germination, during which embryo cells switch from a quiescent state to a metabolically active state rapidly. MicroRNAs (miRNAs) have increasingly been shown to play important roles in rice development. Nevertheless, limited knowledge about miRNA regulation has been obtained in the early stages of rice seed germination. In this study, the small RNAs (sRNAs) from embryos of 0, 12, and 24 HAI rice seeds were sequenced to investigate the composition and expression patterns of miRNAs. The bioinformatics analysis identified 289 miRNA loci, including 59 known and 230 novel miRNAs, and 35 selected miRNAs were confirmed by stem-loop real-time RT-PCR. Expression analysis revealed that the dry and imbibed seeds have unique miRNA expression patterns compared with other tissues, particularly for the dry seeds. Using three methods, Mireap, psRNATarget and degradome analyses, 1197 potential target genes of identified miRNAs involved in various molecular functions were predicted. Among these target genes, 39 had significantly negative correlations with their corresponding miRNAs as inferred from published transcriptome data, and 6 inversely expressed miRNA-target pairs were confirmed by 5'-RACE assay. Our work provides an inventory of miRNA expression profiles and miRNA-target interactions in rice embryos, and lays a foundation for further studies of miRNA-mediated regulation in initial seed germination. |
| 资助项目 | National Science Foundation of China[31101213] ; National Science Foundation of China[31271805] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| WOS记录号 | WOS:000366723400074 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/159]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Yang, Pingfang |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Special Agr, Wuhan 430074, Peoples R China 2.Univ Chinese Acad Sci, Beijing 100049, Peoples R China 3.Wuhan Univ, Coll Iife Sci, Wuhan 430072, Peoples R China |
| 推荐引用方式 GB/T 7714 | He, Dongli,Wang, Qiong,Wang, Kun,et al. Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination[J]. PLOS ONE,2015,10(12):21. |
| APA | He, Dongli,Wang, Qiong,Wang, Kun,&Yang, Pingfang.(2015).Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination.PLOS ONE,10(12),21. |
| MLA | He, Dongli,et al."Genome-Wide Dissection of the MicroRNA Expression Profile in Rice Embryo during Early Stages of Seed Germination".PLOS ONE 10.12(2015):21. |
入库方式： OAI收割
来源：武汉植物园
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