Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS
文献类型：期刊论文
| 作者 | Zhu, Mingzhi1,2; Dong, Xia1; Guo, Mingquan1,3 |
| 刊名 | MOLECULES
 |
| 出版日期 | 2015-12-01 |
| 卷号 | 20期号:12页码:22463-22475 |
| 关键词 | Duchesnea indica phenolic profiling HPLC mass spectrometry |
| ISSN号 | 1420-3049 |
| DOI | 10.3390/molecules201219859 |
| 英文摘要 | Duchesnea indica (D. indica) is an important traditional Chinese medicine, and has long been clinically used to treat cancer in Asian countries. It has been described previously as a rich source of phenolic compounds with a broad array of diversified structures, which are the major active ingredients. However, an accurate and complete phenolic profiling has not been determined yet. In the present work, the total phenolic compounds in crude extracts from D. indica were enriched and fractionated over a macroporous resin column, then identified by HPLC-ESI-MS/MS and ESI-IT-MS (ion trap MS). A total of 27 phenolic compounds were identified in D. indica, of which 21 compounds were identified for the first time. These 27 phenolic compounds encompassing four phenolic groups, including ellagitannins, ellagic acid and ellagic acid glycosides, hydroxybenzoic acid and hydroxycinnamic acid derivatives, and flavonols, were then successfully quantified using peak areas against those of the corresponding standards with good linearity (R-2 > 0.998) in the range of the tested concentrations. As a result, the contents of individual phenolic compounds varied from 6.69 mg per 100 g dry weight (DW) for ellagic acid to 71.36 mg per 100 g DW for brevifolin carboxylate. Not only did this study provide the first phenolic profiling of D. indica, but both the qualitative identification and the subsequent quantitative analysis of 27 phenolic compounds from D. indica should provide a good basis for future exploration of this valuable medicinal plant. |
| 资助项目 | Chinese Academy of Sciences[29Y429291a0129] ; Sino-Africa joint research project[SAJC201328] |
| WOS研究方向 | Chemistry |
| 语种 | 英语 |
| WOS记录号 | WOS:000367533400110 |
| 出版者 | MDPI AG |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/170]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Guo, Mingquan |
| 作者单位 | 1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 2.Macau Univ Sci & Technol, Macau Inst Appl Res Med & Hlth, State Key Lab Qual Res Chinese Med, Macau, Peoples R China 3.Chinese Acad Sci, Sino Africa Joint Res Ctr, Wuhan 430074, Peoples R China |
| 推荐引用方式 GB/T 7714 | Zhu, Mingzhi,Dong, Xia,Guo, Mingquan. Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS[J]. MOLECULES,2015,20(12):22463-22475. |
| APA | Zhu, Mingzhi,Dong, Xia,&Guo, Mingquan.(2015).Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS.MOLECULES,20(12),22463-22475. |
| MLA | Zhu, Mingzhi,et al."Phenolic Profiling of Duchesnea indica Combining Macroporous Resin Chromatography (MRC) with HPLC-ESI-MS/MS and ESI-IT-MS".MOLECULES 20.12(2015):22463-22475. |
入库方式： OAI收割
来源：武汉植物园
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