The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes
文献类型：期刊论文
| 作者 | Zhao, Huayan3; Lu, Shiyou6; Li, Ruixi4,5; Chen, Tao3; Zhang, Huoming2; Cui, Peng3; Ding, Feng3; Liu, Pei1; Wang, Guangchao2; Xia, Yiji1 |
| 刊名 | JOURNAL OF EXPERIMENTAL BOTANY
 |
| 出版日期 | 2015-11-01 |
| 卷号 | 66期号:21页码:6863-6875 |
| 关键词 | AtLSG1 Arabidopsis auxin homeostasis expression pattern map-based cloning proteomics ribosome biogenesis |
| ISSN号 | 0022-0957 |
| DOI | 10.1093/jxb/erv391 |
| 英文摘要 | The circularly permuted GTPase large subunit GTPase 1 (LSG1) is involved in the maturation step of the 60S ribosome and is essential for cell viability in yeast. Here, an Arabidopsis mutant dig6 (drought inhibited growth of lateral roots) was isolated. The mutant exhibited multiple auxin-related phenotypes, which included reduced lateral root number, altered leaf veins, and shorter roots. Genetic mapping combined with next-generation DNA sequencing identified that the mutation occurred in AtLSG1-2. This gene was highly expressed in regions of auxin accumulation. Ribosome profiling revealed that a loss of function of AtLSG1-2 led to decreased levels of monosomes, further demonstrating its role in ribosome biogenesis. Quantitative proteomics showed that the expression of certain proteins involved in ribosome biogenesis was differentially regulated, indicating that ribosome biogenesis processes were impaired in the mutant. Further investigations showed that an AtLSG1-2 deficiency caused the alteration of auxin distribution, response, and transport in plants. It is concluded that AtLSG1-2 is integral to ribosome biogenesis, consequently affecting auxin homeostasis and plant development. |
| 资助项目 | King Abdullah University of Science and Technology (KAUST)[OCRF-2014-CRG3-62140394] |
| WOS研究方向 | Plant Sciences |
| 语种 | 英语 |
| WOS记录号 | WOS:000367814500025 |
| 出版者 | OXFORD UNIV PRESS |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/181]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Xiong, Liming |
| 作者单位 | 1.Hong Kong Baptist Univ, Dept Biol, Hong Kong, Hong Kong, Peoples R China 2.King Abdullah Univ Sci & Technol, Biosci Core Lab, Thuwal 239556900, Saudi Arabia 3.King Abdullah Univ Sci & Technol, Div Biol & Environm Sci & Engn, Thuwal 239556900, Saudi Arabia 4.Univ Calif Riverside, Dept Bot & Plant Sci, Riverside, CA 92521 USA 5.Univ Calif Riverside, Inst Integrat Genome Biol, Ctr Plant Cell Biol, Riverside, CA 92521 USA 6.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Peoples R China 7.Univ Louisville, Dept Biol, Louisville, KY 40292 USA |
| 推荐引用方式 GB/T 7714 | Zhao, Huayan,Lu, Shiyou,Li, Ruixi,et al. The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes[J]. JOURNAL OF EXPERIMENTAL BOTANY,2015,66(21):6863-6875. |
| APA | Zhao, Huayan.,Lu, Shiyou.,Li, Ruixi.,Chen, Tao.,Zhang, Huoming.,...&Xiong, Liming.(2015).The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes.JOURNAL OF EXPERIMENTAL BOTANY,66(21),6863-6875. |
| MLA | Zhao, Huayan,et al."The Arabidopsis gene DIG6 encodes a large 60S subunit nuclear export GTPase 1 that is involved in ribosome biogenesis and affects multiple auxin-regulated development processes".JOURNAL OF EXPERIMENTAL BOTANY 66.21(2015):6863-6875. |
入库方式： OAI收割
来源：武汉植物园
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