Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed
文献类型：期刊论文
| 作者 | Sun, Xingchao; Deng, Linbin; Zhang, Liang; Li, Rongjun; Yang, Hongli; Hu, Zhiyong; Hua, Wei; Liu, Jing; Wang, Hanzhong; Wang, Xinfa |
| 刊名 | PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
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| 出版日期 | 2015 |
| 英文摘要 | Seed weight (SW), which is one of the three major factors influencing grain yield, has been widely accepted as a complex trait that is controlled by polygenes, particularly in polyploid crops. Brassica napus L., which is the second leading crop source for vegetable oil around the world, is a tetraploid (4x) species. In the present study, we identified a major quantitative trait locus (QTL) on chromosome A9 of rapeseed in which the genes for SW and silique length (SL) were colocated. By fine mapping and association analysis, we uncovered a 165-bp deletion in the auxin-response factor 18 (ARF18) gene associated with increased SW and SL. ARF18 encodes an auxin-response factor and shows inhibitory activity on downstream auxin genes. This 55-aa deletion prevents ARF18 from forming homodimers, in turn resulting in the loss of binding activity. Furthermore, reciprocal crossing has shown that this QTL affects SW by maternal effects. Transcription analysis has shown that ARF18 regulates cell growth in the silique wall by acting via an auxin-response pathway. Together, our results suggest that ARF18 regulates silique wall development and determines SW via maternal regulation. In addition, our study reveals the first (to our knowledge) QTL in rapeseed and may provide insights into gene cloning involving polyploid crops. |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/207]  |
| 专题 | 中国科学院武汉植物园 |
| 推荐引用方式 GB/T 7714 | Sun, Xingchao,Deng, Linbin,Zhang, Liang,et al. Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA,2015. |
| APA | Sun, Xingchao.,Deng, Linbin.,Zhang, Liang.,Li, Rongjun.,Yang, Hongli.,...&Wang, Xinfa.(2015).Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed.PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. |
| MLA | Sun, Xingchao,et al."Natural variation in ARF18 gene simultaneously affects seed weight and silique length in polyploid rapeseed".PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA (2015). |
入库方式： OAI收割
来源：武汉植物园
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