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Discovery and comparative assessment of SNPs and SSRs for genetic diversity analysis of wild Heritiera littoralis Dryand (Sterculiaceae), an endangered mangrove tree species

文献类型：期刊论文

作者Wang, Shengwei1,3; Wang, Ying1; Jian, Shuguang2; Hu, Weiming1,3
刊名CONSERVATION GENETICS RESOURCES
出版日期2015-09-01
卷号7期号:3页码:663-668
关键词Heritiera littoralis SNP markers SSR markers Transcriptome sequencing
ISSN号1877-7252
DOI10.1007/s12686-015-0472-0
英文摘要The looking-glass mangrove, Heritiera littoralis Dryand (Sterculiaceae), is an endangered mangrove tree species. Understanding population genetic structure based on molecular markers is essential for the conservation of this ecologically important tree species. Here we identified a total of 1393 potential single nucleotide polymorphism (SNP) and 433 simple sequence repeat (SSR) markers, respectively, using comprehensive transcriptome analysis of H. littoralis. Thirty-six SNPs were subsequently selected for validation using cleaved amplified polymorphic sequences (CAPS) and 19 were confirmed. 16 out of 24 selected SSR markers were also confirmed by fluorescent capillary electrophoresis. Genetic parameters of these validated SNP and SSR markers were then examined in 48 individuals from two wild populations. This first set of SNPs and SSRs developed from transcriptome sequences will benefit the genetic diversity assessment of this species.
资助项目Chinese Academy of Sciences[YZ201227] ; National Natural Science Funds of China[30870244]
WOS研究方向Biodiversity & Conservation ; Genetics & Heredity
语种英语
WOS记录号WOS:000359521500014
出版者SPRINGER
源URL[http://202.127.146.157/handle/2RYDP1HH/214]  
专题中国科学院武汉植物园
通讯作者Wang, Ying
作者单位1.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China
2.Chinese Acad Sci, South China Bot Garden, Key Lab Vegetat Restorat & Management Degraded Ec, Guangzhou 510650, Guangdong, Peoples R China
3.Univ Chinese Acad Sci, Beijing 100049, Peoples R China
推荐引用方式
GB/T 7714		 Wang, Shengwei,Wang, Ying,Jian, Shuguang,et al. Discovery and comparative assessment of SNPs and SSRs for genetic diversity analysis of wild Heritiera littoralis Dryand (Sterculiaceae), an endangered mangrove tree species[J]. CONSERVATION GENETICS RESOURCES,2015,7(3):663-668.
APA Wang, Shengwei,Wang, Ying,Jian, Shuguang,&Hu, Weiming.(2015).Discovery and comparative assessment of SNPs and SSRs for genetic diversity analysis of wild Heritiera littoralis Dryand (Sterculiaceae), an endangered mangrove tree species.CONSERVATION GENETICS RESOURCES,7(3),663-668.
MLA Wang, Shengwei,et al."Discovery and comparative assessment of SNPs and SSRs for genetic diversity analysis of wild Heritiera littoralis Dryand (Sterculiaceae), an endangered mangrove tree species".CONSERVATION GENETICS RESOURCES 7.3(2015):663-668.

入库方式： OAI收割

来源：武汉植物园

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