Gene Expression Profiling of Development and Anthocyanin Accumulation in Kiwifruit (Actinidia chinensis) Based on Transcriptome Sequencing
文献类型：期刊论文
| 作者 | Huang, Hongwen3,5; Li, Wenbin1,2,3,4; Liu, Yifei5; Zeng, Shaohua5; Xiao, Gong3; Wang, Gan1,4; Wang, Ying3; Peng, Ming4 |
| 刊名 | PLOS ONE
 |
| 出版日期 | 2015-08-24 |
| 卷号 | 10期号:8页码:19 |
| ISSN号 | 1932-6203 |
| DOI | 10.1371/journal.pone.0136439 |
| 英文摘要 | Red-fleshed kiwifruit (Actinidia chinensis Planch. 'Hongyang') is a promising commercial cultivar due to its nutritious value and unique flesh color, derived from vitamin C and anthocyanins. In this study, we obtained transcriptome data of 'Hongyang' from seven developmental stages using Illumina sequencing. We mapped 39-54 million reads to the recently sequenced kiwifruit genome and other databases to define gene structure, to analyze alternative splicing, and to quantify gene transcript abundance at different developmental stages. The transcript profiles throughout red kiwifruit development were constructed and analyzed, with a focus on the biosynthesis and metabolism of compounds such as phytohormones, sugars, starch and L-ascorbic acid, which are indispensable for the development and formation of quality fruit. Candidate genes for these pathways were identified through MapMan and phylogenetic analysis. The transcript levels of genes involved in sucrose and starch metabolism were consistent with the change in soluble sugar and starch content throughout kiwifruit development. The metabolism of L-ascorbic acid was very active, primarily through the L-galactose pathway. The genes responsible for the accumulation of anthocyanin in red kiwifruit were identified, and their expression levels were investigated during kiwifruit development. This survey of gene expression during kiwifruit development paves the way for further investigation of the development of this uniquely colored and nutritious fruit and reveals which factors are needed for high quality fruit formation. This transcriptome data and its analysis will be useful for improving kiwifruit genome annotation, for basic fruit molecular biology research, and for kiwifruit breeding and improvement. |
| 资助项目 | National Natural Science Foundation of China[30900119] ; Key Laboratory of Plant Conservation and Sustainable Utilization of SCBG-CAS[211006] ; major technology project of Hainan[ZDZX2013023-1] ; National Natural Science Foundation[31471847] |
| WOS研究方向 | Science & Technology - Other Topics |
| 语种 | 英语 |
| WOS记录号 | WOS:000359951900069 |
| 出版者 | PUBLIC LIBRARY SCIENCE |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/217]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Wang, Ying |
| 作者单位 | 1.Minist Agr, Key Lab Biol & Genet Resources Trop Crops, Haikou, Peoples R China 2.Univ Chinese Acad Sci, Beijing, Peoples R China 3.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan, Hubei, Peoples R China 4.Chinese Acad Trop Agr Sci, Inst Trop Biosci & Biotechnol, Haikou, Hainan, Peoples R China 5.Chinese Acad Sci, South China Bot Garden, Key Lab Plant Resources Conservat & Sustainable U, Guangzhou, Guangdong, Peoples R China |
| 推荐引用方式 GB/T 7714 | Huang, Hongwen,Li, Wenbin,Liu, Yifei,et al. Gene Expression Profiling of Development and Anthocyanin Accumulation in Kiwifruit (Actinidia chinensis) Based on Transcriptome Sequencing[J]. PLOS ONE,2015,10(8):19. |
| APA | Huang, Hongwen.,Li, Wenbin.,Liu, Yifei.,Zeng, Shaohua.,Xiao, Gong.,...&Peng, Ming.(2015).Gene Expression Profiling of Development and Anthocyanin Accumulation in Kiwifruit (Actinidia chinensis) Based on Transcriptome Sequencing.PLOS ONE,10(8),19. |
| MLA | Huang, Hongwen,et al."Gene Expression Profiling of Development and Anthocyanin Accumulation in Kiwifruit (Actinidia chinensis) Based on Transcriptome Sequencing".PLOS ONE 10.8(2015):19. |
入库方式： OAI收割
来源：武汉植物园
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