Development of Microsatellite Markers in Tung Tree (Vernicia fordii) Using Cassava Genomic Sequences
文献类型：期刊论文
| 作者 | You, Frank M.4; Peng, Junhua1; Sun, Dongfa6; Lu, Shiyou2; Nevo, Eviatar5; Luo, Mingcheng3; Zhang, Lingling2 |
| 刊名 | PLANT MOLECULAR BIOLOGY REPORTER
 |
| 出版日期 | 2015-08-01 |
| 卷号 | 33期号:4页码:893-904 |
| ISSN号 | 0735-9640 |
| 关键词 | Energy plant Tung tree Cassava SSR marker Transferability |
| DOI | 10.1007/s11105-014-0804-3 |
| 英文摘要 | Tung tree, Vernicia fordii, is a native oil-bearing woody plant of China. Oil extracted from the fruit is an important industrial and biodiesel feedstock. Microsatellite (simple-sequence repeat (SSR)) markers are effective in germplasm evaluation and marker-assisted breeding of plants. The objective of this study was to develop SSR markers in tung tree using cassava genomic sequences and test the effectiveness of the interspecific transferable SSR markers. According to the principle of comparative genomics, SSR markers were designed based on cassava genomic sequences and tested in tung tree and other Euphorbiaceae plant species. Among 255 cassava SSR markers, 104 (41 %) could effectively amplify DNA of tung tree, and a large portion of these transferable SSR markers could work well in other Euphorbiaceae species, Vernicia montana (98 %), castor bean (92 %), and Jatropha curcas (88 %). Genotyping a set of 16 tung tree accessions using the 104 transferable SSRs, a total of 437 alleles were obtained, 162 of which were polymorphic, and the Shannon information index varied from 0.000 to 0.560 with a mean of 0.139. A phylogenetic tree unambiguously distinguished the 32 accessions of the abovementioned five Euphorbiaceae species. Using cassava genomics resources is an effective approach to develop SSR markers for tung tree when its genome sequence is not available. The developed SSR markers should facilitate germplasm evaluation and other molecular-level investigations in tung tree. |
| 资助项目 | National Natural Science Foundation of China (NSFC)[31030055] ; National Natural Science Foundation of China (NSFC)[30870233] ; Chinese Academy of Sciences[KSCX2-YW-Z-0722] |
| WOS研究方向 | Biochemistry & Molecular Biology ; Plant Sciences |
| 语种 | 英语 |
| 出版者 | SPRINGER |
| WOS记录号 | WOS:000360392400013 |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/225]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Peng, Junhua |
| 作者单位 | 1.Colorado State Univ, Dept Soil & Crop Sci, Ft Collins, CO 80523 USA 2.Chinese Acad Sci, Wuhan Bot Garden, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan 430074, Hubei, Peoples R China 3.Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA 4.Agr & Agri Food Canada, Winnipeg, MB R3T 2M9, Canada 5.Univ Haifa, Inst Evolut, IL-31905 Haifa, Israel 6.Huazhong Agr Univ, Coll Plant Sci & Technol, Wuhan 430071, Hubei, Peoples R China |
| 推荐引用方式 GB/T 7714 | You, Frank M.,Peng, Junhua,Sun, Dongfa,et al. Development of Microsatellite Markers in Tung Tree (Vernicia fordii) Using Cassava Genomic Sequences[J]. PLANT MOLECULAR BIOLOGY REPORTER,2015,33(4):893-904. |
| APA | You, Frank M..,Peng, Junhua.,Sun, Dongfa.,Lu, Shiyou.,Nevo, Eviatar.,...&Zhang, Lingling.(2015).Development of Microsatellite Markers in Tung Tree (Vernicia fordii) Using Cassava Genomic Sequences.PLANT MOLECULAR BIOLOGY REPORTER,33(4),893-904. |
| MLA | You, Frank M.,et al."Development of Microsatellite Markers in Tung Tree (Vernicia fordii) Using Cassava Genomic Sequences".PLANT MOLECULAR BIOLOGY REPORTER 33.4(2015):893-904. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。