Proteomic Analysis of Phosphoproteins in the Rice Nucleus During the Early Stage of Seed Germination
文献类型：期刊论文
| 作者 | Yang, Pingfang2; Sakata, Katsumi3; Yin, Xiaojian1; Li, Ming1,2; Komatsu, Setsuko1 |
| 刊名 | JOURNAL OF PROTEOME RESEARCH
 |
| 出版日期 | 2015-07-01 |
| 卷号 | 14期号:7页码:2884-2896 |
| 关键词 | rice nucleus phosphoproteomics germination phosphonetwork |
| ISSN号 | 1535-3893 |
| 英文摘要 | The early stage of seed germination is the first step in the plant life cycle without visible morphological change, To investigate the mechanism controlling the early stage of rice seed germination, we performed gel-and label free nuclear phosphoproteomics. A total of 3467 phosphopeptides belonging to 102 nuclear phosphoproteins from rice embryos were identified. Protein-synthesisrelated proteins were mainly phosphorylated. During the first 24 h following imbibition, 115 nuclear phosphoproteins were identified, and significant changes in the phosphorylation level over time were observed in 29 phOsphoproteins. Cluster analysis indicated that nucleotide-binding proteins and zinc finger CCCH- and BED-type proteins increased in abundance during the first 12 h of imbibition and then decreased. The in silk protein-protein interactions for 29 nuclear phosphoproteins indicated that the Sas10/Utp3 protein, which functions in snoRNA binding and gene silencing, was the center of the phosphoprotein network in nuclei. The germination rate of seeds was significantly slowed with phosphatase inhibitor treatment. The mRNA expression of the zinc finger CCCH-type protein did not change, and the zinc finger BED-type protein was upregulated in rice embryos during the early stage of germination with phosphatase inhibitor treatthent. These results suggest that the phosphorylation and dephosphorylation of nuclear proteins are involved in rice seed germination. Furthermore, transcription factors such as zinc finger CCCH- and BED-type proteins might play a key role through nuclear phosphoproteins, and Sas10/Utp3 protein might interact with nuclear phosphoproteins in rice embryos to mediate the early stage of seed germination. |
| 资助项目 | National Natural Science Foundation of China[31271805] ; National Agriculture and Food Research Organization |
| WOS研究方向 | Biochemistry & Molecular Biology |
| 语种 | 英语 |
| WOS记录号 | WOS:000357624400014 |
| 出版者 | AMER CHEMICAL SOC |
| 源URL | [http://202.127.146.157/handle/2RYDP1HH/243]  |
| 专题 | 中国科学院武汉植物园 |
| 通讯作者 | Komatsu, Setsuko |
| 作者单位 | 1.Natl Inst Crop Sci, Natl Agr & Food Res Org, Tsukuba, Ibaraki 3058518, Japan 2.Chinese Acad Sci, Key Lab Plant Germplasm Enhancement & Specialty A, Wuhan Bot Garden, Wuhan 430074, Peoples R China 3.Maebashi Inst Technol, Dept Life Sci & Informat, Maebashi, Gumma 3710816, Japan |
| 推荐引用方式 GB/T 7714 | Yang, Pingfang,Sakata, Katsumi,Yin, Xiaojian,et al. Proteomic Analysis of Phosphoproteins in the Rice Nucleus During the Early Stage of Seed Germination[J]. JOURNAL OF PROTEOME RESEARCH,2015,14(7):2884-2896. |
| APA | Yang, Pingfang,Sakata, Katsumi,Yin, Xiaojian,Li, Ming,&Komatsu, Setsuko.(2015).Proteomic Analysis of Phosphoproteins in the Rice Nucleus During the Early Stage of Seed Germination.JOURNAL OF PROTEOME RESEARCH,14(7),2884-2896. |
| MLA | Yang, Pingfang,et al."Proteomic Analysis of Phosphoproteins in the Rice Nucleus During the Early Stage of Seed Germination".JOURNAL OF PROTEOME RESEARCH 14.7(2015):2884-2896. |
入库方式： OAI收割
来源：武汉植物园
浏览0
下载0
收藏0
其他版本
除非特别说明，本系统中所有内容都受版权保护，并保留所有权利。